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dc.contributor.authorBakker, Judith
dc.contributor.authorWangensteen Fuentes, Owen Simon
dc.contributor.authorBaillie, Charles
dc.contributor.authorBuddo, Dayne
dc.contributor.authorChapman, Demian D.
dc.contributor.authorGallagher, Austin J.
dc.contributor.authorGuttridge, Tristan L.
dc.contributor.authorHertler, Heidi
dc.contributor.authorMariani, Stefano
dc.description.abstractOur understanding of marine communities and their functions in an ecosystem relies on the ability to detect and monitor species distributions and abundances. Currently, the use of environmental DNA (eDNA) metabarcoding is increasingly being applied for the rapid assessment and monitoring of aquatic species. Most eDNA metabarcoding studies have either focussed on the simultaneous identification of a few specific taxa/groups or have been limited in geographical scope. Here, we employed eDNA metabarcoding to compare beta diversity patterns of complex pelagic marine communities in tropical coastal shelf habitats spanning the whole Caribbean Sea. We screened 68 water samples using a universal eukaryotic COI barcode region and detected highly diverse communities, which varied significantly among locations, and proved good descriptors of habitat type and environmental conditions. Less than 15% of eukaryotic taxa were assigned to metazoans, most DNA sequences belonged to a variety of planktonic “protists,” with over 50% of taxa unassigned at the phylum level, suggesting that the sampled communities host an astonishing amount of micro‐eukaryotic diversity yet undescribed or absent from COI reference databases. Although such a predominance of micro‐eukaryotes severely reduces the efficiency of universal COI markers to investigate vertebrate and other metazoans from aqueous eDNA, the study contributes to the advancement of rapid biomonitoring methods and brings us closer to a full inventory of extant marine biodiversity.en_US
dc.identifier.citationBakker J, Wangensteen Fuentes OS, Baillie, Buddo D, Chapman DD, Gallagher AJ, Guttridge TL, Hertler H, Mariani S. Biodiversity assessment of tropical shelf eukaryotic communities via pelagic eDNA metabarcoding. Ecology and Evolution. 2019en_US
dc.identifier.cristinIDFRIDAID 1759540
dc.relation.journalEcology and Evolution
dc.rights.holder© The Authorsen_US
dc.rightsAttribution 4.0 International*
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecology: 488en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økologi: 488en_US
dc.titleBiodiversity assessment of tropical shelf eukaryotic communities via pelagic eDNA metabarcodingen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US

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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International