Comparison of cytokine changes in three different lipoprotein apheresis systems in an ex vivo whole blood model
AuthorHardersen, Randolf Inge; Enebakk, Terje; Christiansen, Dorte; Ludviksen, Judith K; Mollnes, Tom Eirik; Lappegård, Knut Tore; Hovland, Anders
Methods - We evaluated how whole blood adsorption, dextran sulfate plasma adsorption, and double filtration plasmapheresis lipoprotein apheresis systems affected cytokine concentrations, using a human whole blood ex vivo model differentiating the effect of the lipoprotein apheresis and plasma separation columns and describing temporal changes.
Results - Compared to the control bag, the whole blood adsorption system reduced Interferon‐γ (IFN‐γ), IL‐8, IL‐1ra, eotaxin, tumor necrosis factor (TNF), monocyte chemoattractant protein 1 (MCP‐1), platelet derived growth factor (PDGF)‐BB, regulated on activation T cell expressed and secreted (RANTES), macrophage inflammatory protein‐1β (MIP‐1β), and IP‐10 (P < .05). The dextran sulfate plasma adsorption system reduced IFN‐γ, IL‐8, IL‐1ra, eotaxin, TNF, MCP‐1, PDGF‐BB, MIP‐1β, and IP‐10 (P < .05). Vascular endothelial growth factor (VEGF) and granulocyte macrophage colony stimulating factor (GM‐CSF) were increased in the whole blood and dextran sulfate plasma adsorption systems (P < .05). The double filtration plasmapheresis system reduced IFN‐γ, IL‐1ra, TNF, MIP‐1β, and IP‐10 (P < .05), while MCP‐1,VEGF, GM‐CSF, and RANTES were increased (P < .05). The plasma separation column increased concentration of RANTES, and was a barrier to reduction of eotaxin. Temporal patterns of concentration change indicated first pass increase of PDGF‐BB and first pass reduction of IP‐10.
Conclusion - There were marked differences in how the three systems affected total and temporal cytokine concentration changes in this in vitro model, as well as compared to former in vivo studies.