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dc.contributor.authorHolt, Margrethe Flesvig
dc.contributor.authorSeim, Bjørn Edvard
dc.contributor.authorØgaard, Jonas
dc.contributor.authorOlsen, Maria Belland
dc.contributor.authorWoldbæk, Per R
dc.contributor.authorKvitting, John Peder Escobar
dc.contributor.authorAukrust, Pål
dc.contributor.authorYndestad, Arne
dc.contributor.authorMollnes, Tom Eirik
dc.contributor.authorNilsson, Per
dc.contributor.authorLouwe, Maria Cornelia
dc.contributor.authorRanheim, Trine
dc.date.accessioned2020-03-04T13:42:53Z
dc.date.available2020-03-04T13:42:53Z
dc.date.issued2019-11-10
dc.description.abstract<i>Objective</i> - The aetiology of thoracic aortic aneurysm (TAA) is largely unknown, but inflammation is likely to play a central role in the pathogenesis. In this present study, we aim to investigate the complement receptors in TAA.<p> <p><i>Methods</i> - Aortic tissue and blood from 31 patients with non-syndromic TAA undergoing thoracic aortic repair surgery were collected. Aortic tissue and blood from 36 patients with atherosclerosis undergoing coronary artery bypass surgery or aortic valve replacement were collected and served as control material. The expression of the complement anaphylatoxin receptors C3aR1, C5aR1 and C5aR2 in aortic tissue were examined by quantitative RT-PCR and C5aR2 protein by immunohistochemistry. Colocalisation of C5aR2 to different cell types was analysed by immunofluorescence. Complement activation products C3bc and sC5b-9 were measured in plasma.<p> <p><i>Results</i> - Compared with controls, TAA patients had substantial (73%) downregulated gene expression of C5aR2 as seen both at the mRNA (p=0.005) level and protein (p=0.03) level. In contrast, there were no differences in the expression of C3aR1 and C5aR1 between the two groups. Immunofluorescence examination showed that C5aR2 was colocalised to macrophages and T cells in the aortic media. There were no differences in the degree of systemic complement activation between the two groups.<p> <p><i>Conclusion</i> - Our findings suggest downregulation of the C5aR2, regarded to act mainly anti-inflammatory, in electively operated TAA as compared with non-aneurysmatic aortas of patients with aortic stenosis and/or coronary artery disease. This may tip the balance towards a relative increase in the inflammatory responses induced by C5aR1 and thus enhance the inflammatory processes in TAA.en_US
dc.identifier.citationHolt MF, Seim Be, Øgaard J, Olsen M, Woldbæk PR, Kvitting JPE, Aukrust P, Yndestad A, Mollnes TE, Nilsson P, Louwe MC, Ranheim T. Selective and marked decrease of complement receptor C5aR2 in human thoracic aortic aneurysms: A dysregulation with potential inflammatory effects. Open heart. 2019;6.e001098(2):1-8en_US
dc.identifier.cristinIDFRIDAID 1757174
dc.identifier.doi10.1136/openhrt-2019-001098
dc.identifier.issn2053-3624
dc.identifier.urihttps://hdl.handle.net/10037/17614
dc.language.isoengen_US
dc.publisherBMJ Publishing Groupen_US
dc.relation.journalOpen heart
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.titleSelective and marked decrease of complement receptor C5aR2 in human thoracic aortic aneurysms: A dysregulation with potential inflammatory effectsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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