Adaptive fluctuation imaging captures rapid subcellular dynamics

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https://hdl.handle.net/10037/17740
DOI
https://doi.org/10.1117/12.2526846
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Date
2019-07-22
Type
Journal article
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Author
Opstad, Ida Sundvor; Ströhl, Florian; Birgisdottir, Åsa birna; Acuña Maldonado, Sebastian Andres; Kalstad, Trine; Myrmel, Truls; Agarwal, Krishna; Ahluwalia, Balpreet Singh
Abstract
In this work we have explored the live-cell friendly nanoscopy method Multiple Signal Classification Algorithm (MUSICAL) for multi-colour imaging of various organelles and sub-cellular structures in the cardiomyoblast cell line H2c9. We have tested MUSICAL for fast (up to 230Hz), multi-colour time-lapse sequences of various sub-cellular structures (mitochondria, endoplasmic reticulum, microtubules, endosomes and nuclei) in living cells using low excitation-light dose. Challenges and opportunities with applying MUSICAL for studying rapid sub-cellular dynamics are discussed.
Is part of
Opstad, I.S. (2021). Bringing optical nanoscopy to life - Super-resolution microscopy of living cells. (Doctoral thesis). https://hdl.handle.net/10037/20306
Publisher
Society of Photo-Optical Instrumentation Engineers (SPIE)
Citation
Opstad, I.S., Ströhl, F., Birgisdottir, Å.b., Maldonado, S.A.A., Kalstad, T., Myrmel, T., Agarwal, K., Ahluwalia, B.S. (2019) Adaptive fluctuation imaging captures rapid subcellular dynamics. Proceedings of SPIE, the International Society for Optical Engineering, Advances in Microscopic Imaging II, 11076,1-3
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© SPIE. (2019) Society of Photo-Optical Instrumentation Engineers, SPIE