ub.xmlui.mirage2.page-structure.muninLogoub.xmlui.mirage2.page-structure.openResearchArchiveLogo
    • EnglishEnglish
    • norsknorsk
  • Velg spraaknorsk 
    • EnglishEnglish
    • norsknorsk
  • Administrasjon/UB
Vis innførsel 
  •   Hjem
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Norges fiskerihøgskole
  • Artikler, rapporter og annet (Norges fiskerihøgskole)
  • Vis innførsel
  •   Hjem
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Norges fiskerihøgskole
  • Artikler, rapporter og annet (Norges fiskerihøgskole)
  • Vis innførsel
JavaScript is disabled for your browser. Some features of this site may not work without it.

Distinct Host–Mycobacterial Pathogen Interactions between Resistant Adult and Tolerant Tadpole Life Stages of Xenopus laevis

Permanent lenke
https://hdl.handle.net/10037/18000
DOI
https://doi.org/10.4049/jimmunol.1900459
Thumbnail
Åpne
article.pdf (4.408Mb)
Innsendt manusversjon (PDF)
Dato
2019-11-15
Type
Journal article
Tidsskriftartikkel

Forfatter
Rhoo, Kun Hyoe; Edholm, Eva-Stina Isabella; Forzan, Maria J; Khan, Adil Mahboob; Waddle, Anthony W; Pavelka, Martin S; Robert, Jacques
Sammendrag
Mycobacterium marinum is a promiscuous pathogen infecting many vertebrates, including humans, whose persistent infections are problematic for aquaculture and public health. Among unsettled aspects of host–pathogen interactions, the respective roles of conventional and innate-like T (iT) cells in host defenses against M. marinum remain unclear. In this study, we developed an infection model system in the amphibian Xenopus laevis to study host responses to M. marinum at two distinct life stages, tadpole and adult. Adult frogs possess efficient conventional T cell–mediated immunity, whereas tadpoles predominantly rely on iT cells. We hypothesized that tadpoles are more susceptible and elicit weaker immune responses to M. marinum than adults. However, our results show that, although anti–M. marinum immune responses between tadpoles and adults are different, tadpoles are as resistant to M. marinum inoculation as adult frogs. M. marinum inoculation triggered a robust proinflammatory CD8+ T cell response in adults, whereas tadpoles elicited only a noninflammatory CD8 negative- and iT cell–mediated response. Furthermore, adult anti–M. marinum responses induced active granuloma formation with abundant T cell infiltration and were associated with significantly reduced M. marinum loads. This is reminiscent of local CD8+ T cell response in lung granulomas of human tuberculosis patients. In contrast, tadpoles rarely exhibited granulomas and tolerated persistent M. marinum accumulation. Gene expression profiling confirmed poor tadpole CD8+ T cell response, contrasting with the marked increase in transcript levels of the anti–M. marinum invariant TCR rearrangement (iVα45-Jα1.14) and of CD4. These data provide novel insights into the critical roles of iT cells in vertebrate antimycobacterial immune response and tolerance to pathogens.
Forlag
American Association of Immunologists
Sitering
Rhoo, Edholm ES, Forzan, Khan AM, Waddle, Pavelka, Robert J. Distinct Host–Mycobacterial Pathogen Interactions between Resistant Adult and Tolerant Tadpole Life Stages of Xenopus laevis. Journal of Immunology. 2019;203(10):2679-2688
Metadata
Vis full innførsel
Samlinger
  • Artikler, rapporter og annet (Norges fiskerihøgskole) [1053]
Copyright © 2019 by The American Association of Immunologists, Inc.

Bla

Bla i hele MuninEnheter og samlingerForfatterlisteTittelDatoBla i denne samlingenForfatterlisteTittelDato
Logg inn

Statistikk

Antall visninger
UiT

Munin bygger på DSpace

UiT Norges Arktiske Universitet
Universitetsbiblioteket
uit.no/ub - munin@ub.uit.no

Tilgjengelighetserklæring