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dc.contributor.authorHansen, Ida Kristine Østnes
dc.contributor.authorIsaksson, Johan
dc.contributor.authorPoth, Aaron G.
dc.contributor.authorØstnes Hansen, Kine
dc.contributor.authorAndersen, Aaron John Christian
dc.contributor.authorRichard, Céline Sarah Marine
dc.contributor.authorBlencke, Hans-Matti
dc.contributor.authorStensvåg, Klara
dc.contributor.authorCraik, David J.
dc.contributor.authorHaug, Tor
dc.date.accessioned2020-05-12T11:03:55Z
dc.date.available2020-05-12T11:03:55Z
dc.date.issued2020-01-12
dc.description.abstractThis study reports the isolation of two novel cysteine-rich antibacterial peptides, turgencin A and turgencin B, along with their oxidized derivatives, from the Arctic marine colonial ascidian <i>Synoicum turgens</i>. The peptides are post-translationally modified, containing six cysteines with an unusual disulfide connectivity of Cys<sup>1</sup>-Cys<sup>6</sup>, Cys<sup>2</sup>-Cys<sup>5</sup>, and Cys<sup>3</sup>-Cys<sup>4</sup> and an amidated C-terminus. Furthermore, the peptides contain methionine residues resulting in the isolation of peptides with different degrees of oxidation. The most potent peptide, turgencin A<sub>Mox1</sub> with one oxidized methionine, displayed antimicrobial activity against both Gram-negative and Gram-positive bacteria with a minimum inhibitory concentration (MIC) as low as 0.4 µM against selected bacterial strains. In addition, the peptide inhibited the growth of the melanoma cancer cell line A2058 (IC<sub>50</sub> = 1.4 µM) and the human fibroblast cell line MRC-5 (IC<sub>50</sub> = 4.8 µM). The results from this study show that natural peptides isolated from marine tunicates have the potential to be promising drug leads.en_US
dc.identifier.citationHansen IK, Isaksson J, Poth, Østnes Hansen KØ, Andersen AJC, Richard CSM, Blencke H, Stensvåg K, Craik DJ, Haug T. Isolation and characterization of antimicrobial peptides with unusual disulfide connectivity from the colonial ascidian Synoicum turgens. Marine Drugs. 2020;18(1)en_US
dc.identifier.cristinIDFRIDAID 1771217
dc.identifier.doihttps://doi.org/10.3390/md18010051
dc.identifier.issn1660-3397
dc.identifier.urihttps://hdl.handle.net/10037/18264
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalMarine Drugs
dc.relation.projectIDNorges forskningsråd: 269425en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BIOTEK2021/269425/Norway/DL: Digital discovery of antimicrobial molecules from marine Arctic resources with reduced risk of triggering resistance//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400en_US
dc.titleIsolation and characterization of antimicrobial peptides with unusual disulfide connectivity from the colonial ascidian Synoicum turgensen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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