Now showing items 1-10 of 10

    • Biochemical characterization of ParI, an orphan C5-DNA methyltransferase from Psychrobacter arcticus 273-4 

      Grgic, Miriam; Williamson, Adele Kim; Bjerga, Gro Elin Kjæreng; Altermark, Bjørn; Leiros, Ingar (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-05-25)
      <p>Cytosine-specific DNA methyltransferases are important enzymes in most living organisms. In prokaryotes, most DNA methyltransferases are members of the type II restriction-modification system where they methylate host DNA, thereby protecting it from digestion by the accompanying restriction endonucleases. DNA methyltransferases can also act as solitary enzymes having important roles in controlling ...
    • Bioprospecting around Arctic islands: Marine bacteria as rich source of biocatalysts 

      De Santi, Concetta; Altermark, Bjørn; de Pascale, Donatella; Willassen, Nils Peder (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-12-11)
      We have investigated the biotechnological potential of Arctic marine bacteria for their ability to produce a broad spectrum of cold-active enzymes. Marine bacteria exhibiting these features are of great interest for both fundamental research and industrial applications. Macrobiota water and sediment samples have been collected during 2010 and 2011expeditions around the Lofoten and Svalbard islands. ...
    • Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries 

      De Santi, Concetta; Altermark, Bjørn; Pierechod, Marcin Miroslaw; Ambrosino, Luca; de Pascale, Donatella; Willassen, Nils Peder (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-01-19)
      Background: The use of metagenomics in enzyme discovery constitutes a powerful approach to access to genomes of unculturable community of microorganisms and isolate novel valuable biocatalysts for use in a wide range of biotechnological and pharmaceutical fields. Results: Here we present a novel esterase gene (lip3) identified by functional screening of three fosmid metagenomic libraries, ...
    • Complete genome sequence of Halomonas sp. R5-57 

      Williamson, Adele Kim; De Santi, Concetta; Altermark, Bjørn; Karlsen, Christian; Hjerde, Erik (Journal article; Tidsskriftartikkel; Peer reviewed, 2016)
      The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, Halomonas sp. R5-57 exhibits high salt tolerance over a wide range of temperatures and has extra-cellular hydrolytic activities with several ...
    • Features and structure of a cold active N-acetylneuraminic acid lyase 

      Gurung, Man Kumari; Altermark, Bjørn; Helland, Ronny; Smalås, Arne O.; Ræder, Inger Lin Uttakleiv (Journal article; Peer reviewed, 2019-06-11)
      N-acetylneuraminate lyases (NALs) are enzymes that catalyze the reversible cleavage and synthesis of sialic acids. They are therefore commonly used for the production of these high-value sugars. This study presents the recombinant production, together with biochemical and structural data, of the NAL from the psychrophilic bacterium Aliivibrio salmonicida LFI1238 (AsNAL). Our characterization shows ...
    • High quality draft genome sequence of Streptomyces sp. strain AW19M42 isolated from a sea squirt in Northern Norway 

      Bjerga, Gro Elin Kjæreng; Hjerde, Erik; De Santi, Concetta; Williamson, Adele Kim; Smalås, Arne O.; Willassen, Nils Peder; Altermark, Bjørn (Journal article; Tidsskriftartikkel; Peer reviewed, 2014)
    • Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria 

      Strand, Mårten; Carlsson, Marcus; Uvell, Hanna; Islam, Koushikul; Edlund, Karin; Cullman, Inger; Altermark, Bjørn; Mei, Ya-Fang; Elofsson, Mikael; Willassen, Nils Peder; Wadell, Göran; Almqvist, Fredrik (Journal article; Tidsskriftartikkel; Peer reviewed, 2014)
    • A molecular phylogeny of wood-borers (Teredinidae) from Japanese Tsunami Marine Debris 

      Treneman, Nancy C.; Borges, Luisa M. S.; Shipway, Reuben; Raupach, Michael J.; Altermark, Bjørn; Carlton, James T. (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02)
      The family Teredinidae (shipworms) contains 70-plus species of boring bivalves specialized to live in and digest wood. Traditional means of species identification and taxonomy of this group encounter numerous challenges, often compounded by the diverse and dynamic nature of shipworm ecology and distribution. Modern integrative taxonomic methods are shedding new light on this complex group, from ...
    • Properties and distribution of a metallo-β-lactamase (ALI-1) from the fish pathogen Aliivibrio salmonicida LFI1238 

      Kristiansen, Anders; Grgic, Miriam; Altermark, Bjørn; Leiros, Ingar (Journal article; Tidsskriftartikkel; Peer reviewed, 2015)
      Objectives. To characterize the chromosome-encoded metallo-β-lactamase (MBL) from the psychrophilic, marine fish-pathogenic bacterium Aliivibrio salmonicida LFI1238 and check for the presence of the gene in other Aliivibrio isolates both connected to the fish-farming industry and from the environment. Methods. The MBL gene was cloned and intracellularly expressed in Escherichia coli. Kinetic ...
    • Species diversity and abundance of shipworms (Mollusca: Bivalvia: Teredinidae) in woody marine debris generated by the Great East Japan Earthquake and Tsunami of 2011 

      Treneman, Nancy C.; Carlton, James T.; Borges, Luisa M. S.; Shipway, Reuben; Raupach, Michael; Altermark, Bjørn (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02)
      The Tohoku tsunami of March 2011 ejected a vast amount of debris into the Pacific Ocean. Wood boring shipworms (Bivalvia: Teredinidae) were either already present in, or settled on, the wooden fraction of this debris, offering a unique opportunity to study shipworm diversity in rafted wood of a known origin and time of ocean entry. Lumber and other wood began appearing on Central Pacific (Hawaiian ...