Blar i forfatter Mastergradsoppgaver i farmasi "Moen, Merete Kveli"
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Determination of Cysteine Connectivity in Bioactive Peptides
Moen, Merete Kveli (Master thesis; Mastergradsoppgave, 2014-05-20)Conotoxins are diverse and well-studied, and is therefore suited as a model to develop a general method for analyzing disulfide-rich peptides. To investigate the folding of both commercially available and self-synthesized Conotoxins, an UHPLC-MS/MS method was developed where one disulfide-bridge was reduced at the time and alkylated with different maleimides. The results from the UHPLC-MS/MS method ...