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norskMastergradsoppgaver i biologi (Helsefak): Recent submissions
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Modulation of dendritic cells phenotype and functions by irradiated and non-irradiated lung cancer fibroblasts. An in vitro study.
(Master thesis; Mastergradsoppgave, 2019-05-15)Stromal cells in the tumor microenvironment (TME) interacts with cancer cells and affect their behavior in diverse manners. In this study, we investigated in vitro the mechanisms by which non-irradiated and irradiated cancer-associated fibroblasts (CAFs) isolated from patients with non-small cell lung carcinoma (NSCLC) affected the phenotype and function of monocyte-derived dendritic cells (Mo-DCs) ... -
Exploring the in vitro expansion of CD4 T cells. For improved culturing of CD4 T cells linked to FNAIT
(Master thesis; Mastergradsoppgave, 2018-05-15)Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a rare disease that may cause serious bleedings in the fetus or neonate of a woman who has developed antibodies against the fetus’ platelets. Development of FNAIT has been linked to the presence of platelet reactive CD4 T cells that help B cells to develop into antibody producing plasma cells. To be able to conduct research on such T cells, ... -
Identification of protein interaction candidates for the GOLD domain of FYCO1
(Master thesis; Mastergradsoppgave, 2016-05-27)Autophagy is an evolutionary conserved degradative pathway, where damaged or surplus cytosolic components are sequestered into double membrane vesicles, autophagosomes, which become degraded through the lysosomal system. The autophagy is a dynamic process, which is depended of transport of autophagosomes along microtubule, to become degraded by lysosomes. One of the proteins involved in this transport ... -
Forensic DNA genotyping by means of next generation sequencing. Analysis of Autosomal STRs of a Norwegian population sample using the ForenSeq FGx system
(Master thesis; Mastergradsoppgave, 2018-05-15)Population databases containing allele frequencies of the genetic markers used for DNA-profiling are necessary for forensic geneticist to be able to perform statistical calculations on the statistical weight of DNA-evidence. However, allele frequencies differ from population to population, it is therefore important to establish population databases for specific graphical areas or population groups. ... -
The Immunostimulatory Potential of Mesenchymal Stem Cells for formation of Tertiary Lymphoid Structures in Lupus Nephritis
(Master thesis; Mastergradsoppgave, 2017-05-15)Formation of tertiary lymphoid structures (TLS) occurs in tissues targeted by chronic inflammatory processes, such as infection and autoimmunity. In systemic lupus erythematosus (SLE), TLS have been observed in the kidneys of lupus-prone mice and in kidney biopsies of SLE patients with Lupus Nephritis (LN). Here the role of tissue-specific mesenchymal stem cells (MSCs) as lymphoid tissue organizer ... -
The population structure of human carriage and clinical isolates of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Norway
(Master thesis; Mastergradsoppgave, 2017-05-15)The increasing emergence of antimicrobial resistant bacteria worldwide is recognised as a severe threat to public health on a global scale. Without effective antimicrobial agents to treat bacterial infections, modern medicine will be set back several decades and deaths caused by bacterial infections will increase. The most widely used class of antimicrobials, is -lactams, and the increase in resistance ... -
Computational study of SERT using new X-ray crystal structures and initial experimental verification of potential GABAB receptor compounds
(Master thesis; Mastergradsoppgave, 2018-05-15)Depression is currently one of the leading causes worldwide of suicide and disability, the most common treatment is antidepressants. Most antidepressants work by increasing the monoamine levels in the central nervous system by inhibition of the reuptake of monoamines into the presynaptic neuron, and thereby ensure accumulation of the monoamines in the synaptic cleft. The main target for most ... -
“EFFECT OF IRRADIATION ON THE IMMUNOREGULATORY FUNCTIONS EXERTED BY TUMOUR FIBROBLASTS ON MACROPHAGES”
(Master thesis; Mastergradsoppgave, 2018-11-19)Summary Background/aim of the study: Carcinoma-associated fibroblasts (CAFs) represents a heterogeneous population of cells and are considered one of the dominant stromal component of solid tumours, performing a crucial role in tumour proliferation and metastasis. The main objective of this study was to investigate the immunoregulatory features of CAFs isolated from non-small cell lung carcinomas ... -
Characterization of ATG8 Family Protein Binding to TRIM23 and TRIM31
(Master thesis; Mastergradsoppgave, 2018-05-15)Macroautophagy (hereafter called autophagy) is a process where proteins, organelles and intracellular micro-organisms are degraded by the lysosome. The autophagosome engulfs a part of the cytoplasm where the cargo is, and transports it to the endosomal-lysosomal system for degradation. Autophagy can also be selective, where cargos are recognized directly by the autophagy receptors or by other proteins ... -
In vitro reconstitution of proMatrix Metalloproteinase-9/Chondroitin Sulfate Proteoglycan Complexes. Identification of motifs in proMMP-9 and the serglycin core protein involved in the complex formation.
(Master thesis; Mastergradsoppgave, 2014-05-15)Previously it has been shown that different monocytic leukemic cell lines such as THP-1, MonoMac and U-937 can produce proteolytic enzymes such as MMP-9 as well as various types of proteoglycans (PG). These proteins have been shown to be involved in homeostasis as well as in various diseases, such as cancer. When proMMP-9 was mixed with isolated PGs from these three cell lines or pure serglycin and ... -
Hereditary breast and ovarian cancer. Diversity of genetic causes of HBOC in a Norwegian breast and ovarian cancer patient cohort, BRCA2 c.8331+2C>T - a Norwegian founder mutation
(Master thesis; Mastergradsoppgave, 2017-05-14)Hereditary breast and ovarian cancer (HBOC) causes 5-10 % of breast cancer cases and 25 % of ovarian cancer cases. About 24 % of HBOC are caused by deleterious variants in BRCA1 and BRCA2. Currently, more than 25 different genes have been associated with HBOC, including BRCA1 and BRCA2, many of which encode proteins participating in homologous recombination repair (HRR) and mismatch repair (MMR). ... -
The oncoproteins gelsolin, periostin and thrombospondin are enriched in Merkel cell carcinoma exosomes, and their promoter activity is stimulated by Large T-antigen of Merkel cell polyomavirus
(Master thesis; Mastergradsoppgave, 2017-05-14)Merkel cell carcinoma (MCC) is a rare, aggressive neuroendocrine form of skin cancer with high mortality rate and increasing incidence. Around 80 % of MCC tumours are positive for Merkel cell polyomavirus (MCPyV), the only polyomavirus known to induce cancer in humans. Continuous expression of the viral oncogenes LT-ag and st-ag is necessary for MCPyV positive MCC tumour cell survival, suggesting ... -
Analysis of glomerular target structures for nephritic anti-dsDNA antibodies playing central roles in lupus nephritis
(Master thesis; Mastergradsoppgave, 2008-05)Systemic lupus erythematosus is an autoimmune disease characterized by the presence of autoantibodies towards nuclear antigens. Sub-populations of anti-dsDNA antibodies have the potential to bind in the kidney and thereby induce nephritis, but there is no definite distinction that separates non-pathogenic from pathogenic antibodies. There must be parameters that determine anti-dsDNA antibody binding, ... -
Production and purification of recombinant C-terminal truncated pro-Matrix Metalloprotease-9
(Master thesis; Mastergradsoppgave, 2016-06-21)MMP-9 consists of N-terminal signal peptide, a pro-domain, catalytic domain, a long flexible hinge region and C- terminal hemopexin domain. In the present study a truncated version of proMMP-9 which lacking hinge region and C-terminal hemopexin domain was produced. To do so Sf9 insect cells and the BaculoDirect Baculovirus expression system were used. Using site directed mutagenesis, single mutation ... -
Effect of viral early proteins, mutations and IL-17F on the transcriptional activity of the Merkel cell polyomavirus promoter in different cell lines
(Master thesis; Mastergradsoppgave, 2016-05-23)Merkel cell polyomavirus (MCPyV) is common in the human population with a seropositivity of approximately 60%. The virus is chronically shed from healthy skin, but the genuine host cell remains unknown and a permissive cell culture system is lacking. The viral genome is in an episomal state in cells where MCPyV has been found. The virus is not harmful in healthy individuals, but it is involved in ... -
An investigation of Intercellular Adhesion Molecule-1 (ICAM-1) in Breast Cncer
(Master thesis; Mastergradsoppgave, 2016-05-18)Intercellular Adhesion Molecule-1 (ICAM-1, CD54), a cell surface glycoprotein that functions as an integrin, has a well-established role in leukocyte migration during inflammation. Recent studies have indicated a role for this protein in cancer development. In this study three breast cancer cell lines were stimulated with a variety of cytokines. The breast cancer cell lines (MCF7, SK-BR-3 and ... -
Oncolytic Activity and Mechanisms of Action by a small β2,2-amino acid derivative LTX-401
(Master thesis; Mastergradsoppgave, 2015-05-11)Antimicrobial peptides are part of the innate immune defence of many organisms and represent a novel class of therapeutics due to their broad-spectrum activities, including cytotoxic activity against cancer cells. It has recently been reported that small antimicrobial β2,2-amino acid derivatives (Mw<500), such as LTX-401, possess potent anticancer activity against Ramos human Burkitt’s lymphoma ... -
A study of possible genetic causes of inherited breast and ovarian cancer in a Norwegian cancer population
(Master thesis; Mastergradsoppgave, 2013-05-15)Homologous recombination repair (HRR) is an important repair mechanism, and mutations disrupting the function of this machinery might contribute to cancer formation. Several proteins interact in this mechanism, and the two best known are BRCA1 and BRCA2. Mutations in their corresponding genes BRCA1 and BRCA2 are found in 40% of hereditary breast and ovarian cancer cases. However, there are still ... -
Identification, isolation and characterisation of scavenging endothelial cells in placenta
(Master thesis; Mastergradsoppgave, 2014-05-15)Haemopoiesis occurs in the BM, where haemopoietic stem cells (HSC) reside in a three-dimensional “niche” and give rise to the millions of circulating blood cells required everyday of life. The precise regulation of this immense task occurs by interaction of HSC with other BM cells and extracellular matrix molecules. In addition, the BM vasculature has also now been identified as a key HSC regulator. ... -
Role of the mannose receptor in tumor associated macrophages. Studies in a mouse melanoma model
(Master thesis; Mastergradsoppgave, 2014-04-15)Tumor-associated macrophages (TAMs) play an important role in cancer-related inflammation. TAMs develop M2 polarization which may support tumor growth. Mannose receptor (MR) expression is a hallmark for this cell-subpopulation, and the MR has recently been suggested to be involved in TAM polarization. The aim of the study was to investigate the role of MR in TAM polarization and tumor development ...
