Vis enkel innførsel

dc.contributor.advisorVasskog, Terje
dc.contributor.advisorSvenning, Jon Brage
dc.contributor.advisorDalheim, Lars
dc.contributor.authorStokkeland, Ingunn Møgedal
dc.date.accessioned2021-05-22T11:51:06Z
dc.date.available2021-05-22T11:51:06Z
dc.date.issued2019-05-15
dc.description.abstract<p>Background: The Artic University of Norway entered a partnership with a smelting plant to reduce the factory fume CO2-footprint by cultivation of microalgae (diatoms). The biomass produced from the microalgae is rich in lipids, proteins and pigments and can potentially function as fish feed for the aquaculture industry. Before the biomass can be utilized as e.g. fish feed, a thorough investigation of its constituents is important. In this thesis the main goals were to characterize the pigment composition in <i>Porosira glacialis</i> and investigate if different light regimes could affect the pigment composition. <p>Method: The microalgae, <i>Porosira glacialis</i>, cultivated in red, blue and white light regimes was included in the project. From freeze-dried algal biomass, the pigments were extracted with a mixture of methanol and acetone. Two different LC-MS techniques were investigated (Q-orbitrap and Q-TOF) for analyzing extracted pigments. Liquid chromatography coupled to Q Exactive with ESI in full scan mode was applied. A MS/MS mode was used to determine the fragmentation pattern of chlorophyll a and astaxanthin as well as identification of other pigments. <p>Results: Twelve pigments could be detected and identified in <i>P. glacialis</i>, where seven of them are carotenoids. The results suggest that light regimes can regulate the accumulation of different pigments in <i>P. glacialis</i>, especially carotene. The best light regime for accumulating chlorophyll a was white light. <p>Conclusion: The white light regime seems promising in cultivation of the microalgae, <i>P. glacialis</i>, in regards to the amount of pigments. It is however possible to induce a change in relative pigment composition by changing the color of the light during cultivation.en_US
dc.identifier.urihttps://hdl.handle.net/10037/21231
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)en_US
dc.subject.courseIDFAR-3911
dc.subjectFarmasien_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Biopharmacy: 736en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Biofarmasi: 736en_US
dc.titleLC-MS characterization of pigments in microalgae from different cultivation regimesen_US
dc.typeMaster thesisen_US
dc.typeMastergradsoppgaveen_US


Tilhørende fil(er)

Thumbnail
Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)