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dc.contributor.advisorSundsfjord, Arnfinn
dc.contributor.advisorHjerde, Erik
dc.contributor.advisorSamuelsen, Ørjan
dc.contributor.advisorRaffelsberger, Niclas
dc.contributor.authorNilsen, Miriam Kristine
dc.date.accessioned2021-06-07T06:25:15Z
dc.date.available2021-06-07T06:25:15Z
dc.date.issued2020-06-05
dc.description.abstractThe aims were to perform a bioinformatic-statistical analysis of CRISPR-Cas- and R-M systems in a diverse whole genome sequenced K. pneumoniae population, and their correlations to virulence score and AMR -/ plasmid content. The strains (n=999) consisted of Norwegian fecal carrier (n=484) and clinical (NORM; ESBL and non-ESBL producing) (n=414), and national-international clinical ST307 strains (n=101). Structural complete CRISPR-Cas systems were found in 26% of the strains; carrier (30%), NORM non-ESBL (26%), NORM-ESBL (29%), and ST307 (0%). R-M systems were found in 48% of the strains; carrier (43%), NORM (44%) and ST307 (90%). The presence of CRISPR-cas- and R-M systems seems to be equally distributed between carrier and clinical strains. The systems distributions had ST-specific profiles as illustrated with the ST307 strains. Some significant cross-population correlations were observed between the presence/absence of CRISPR-Cas-/R-M systems in terms of MGE acquisition, represented by virulence score, AMR - and plasmid content. CRISPR-Cas systems strains were associated with a higher virulence score and a lower AMR-/plasmid load. The R-M systems strains were associated with lower virulence score and a higher AMR-/plasmid load. Future studies should include analysis of CRISPR spacer content and specificity, overall phage content and utilize more advanced comparisons and statistical analyses.en_US
dc.identifier.urihttps://hdl.handle.net/10037/21361
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)
dc.subject.courseIDMBI-3911
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical microbiology: 715en_US
dc.subjectHorizontal gene transferen_US
dc.subjectRestriction- Modification systemsen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectTromsø7en_US
dc.subjectNORMen_US
dc.subjectNORKABen_US
dc.subjectST307en_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectCRISPR-Cas systemsen_US
dc.titleInvestigation into the presence of CRISPR-Cas- and R-M systems in Klebsiella pneumoniae and correlation with antibiotic resistance, virulence and plasmidsen_US
dc.typeMaster thesisen_US
dc.typeMastergradsoppgaveen_US


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