ub.xmlui.mirage2.page-structure.muninLogoub.xmlui.mirage2.page-structure.openResearchArchiveLogo
    • EnglishEnglish
    • norsknorsk
  • Velg spraaknorsk 
    • EnglishEnglish
    • norsknorsk
  • Administrasjon/UB
Vis innførsel 
  •   Hjem
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Norges fiskerihøgskole
  • Artikler, rapporter og annet (Norges fiskerihøgskole)
  • Vis innførsel
  •   Hjem
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Norges fiskerihøgskole
  • Artikler, rapporter og annet (Norges fiskerihøgskole)
  • Vis innførsel
JavaScript is disabled for your browser. Some features of this site may not work without it.

Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products

Permanent lenke
https://hdl.handle.net/10037/23151
DOI
https://doi.org/10.3390/app112110241
Thumbnail
Åpne
article.pdf (720.9Kb)
Publisert versjon (PDF)
Dato
2021-11-01
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Forfatter
Schneider, Yannik Karl-Heinz; Andersen, Jeanette Hammer; Hansen, Espen; Jørgensen, Solveig M.
Sammendrag
A key step in the process of isolating microbial natural products is the preparation of an extract from a culture. This step determines which molecules will be available for detection in the subsequent chemical and biological analysis of a biodiscovery pipeline. In the present study we wanted to document potential differences in performance between liquid–liquid extraction using ethyl acetate and liquid–solid extraction using a poly‐benzyl‐resin. For the comparison of the two extraction protocols, we spiked a culture of Flavobacterium sp. with a diverse selection of natural products of microbial and plant origin to investigate whether the methods were comparable with respect to selectivity. We also investigated the efficiency of the two extraction methods quantita‐ tively, using water spiked with a selection of natural products, and studied the quantitative effect of different pH levels of the aqueous solutions on the extraction yields of the two methods. The same compounds were extracted by the two methods, but the solid‐phase extract contained more media components compared with the liquid‐phase extract. Quantitatively, the two extraction methods varied in their recovery rates. We conclude that practical aspects could be more important when selecting one of the extraction protocols, as their efficiencies in extracting specific compounds were quite similar.
Sitering
Schneider, Jørgensen S, Andersen, Hansen. Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products. Applied Sciences. 2021;11
Metadata
Vis full innførsel
Samlinger
  • Artikler, rapporter og annet (Norges fiskerihøgskole) [1053]
Copyright 2021 The Author(s)

Bla

Bla i hele MuninEnheter og samlingerForfatterlisteTittelDatoBla i denne samlingenForfatterlisteTittelDato
Logg inn

Statistikk

Antall visninger
UiT

Munin bygger på DSpace

UiT Norges Arktiske Universitet
Universitetsbiblioteket
uit.no/ub - munin@ub.uit.no

Tilgjengelighetserklæring