ub.xmlui.mirage2.page-structure.muninLogoub.xmlui.mirage2.page-structure.openResearchArchiveLogo
    • EnglishEnglish
    • norsknorsk
  • Velg spraaknorsk 
    • EnglishEnglish
    • norsknorsk
  • Administrasjon/UB
Vis innførsel 
  •   Hjem
  • Det helsevitenskapelige fakultet
  • Institutt for farmasi
  • Artikler, rapporter og annet (farmasi)
  • Vis innførsel
  •   Hjem
  • Det helsevitenskapelige fakultet
  • Institutt for farmasi
  • Artikler, rapporter og annet (farmasi)
  • Vis innførsel
JavaScript is disabled for your browser. Some features of this site may not work without it.

Detection of DHCMT long-term metabolite glucuronides with LC-MSMS as an alternative approach to conventional GC-MSMS analysis

Permanent lenke
https://hdl.handle.net/10037/24777
DOI
https://doi.org/10.1016/j.steroids.2022.108979
Thumbnail
Åpne
article.pdf (2.095Mb)
Publisert versjon (PDF)
Dato
2022-02-17
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Forfatter
Göschl, Lorenz; Gmeiner, Günter; Gärtner, Peter; Steinacher, Michael; Forsdahl, Guro
Sammendrag
Dehydrochloromethyltestosterone (DHCMT) is one of the most detected illicit used anabolic–androgenic steroids in professional sports. Therefore, a fast and accurate analysis of this substance is of great importance for a constructive fight against doping abuse. The conventional method for the analysis of this drug, GC-MSMS, is very sensitive and selective but also very time- and resource-consuming. With the presented work, a new approach for simple detection with LC-HRMSMS without any sample preparation is introduced. The method is based on the direct analysis of two newly described phase-II metabolites of the DHCMT long-term metabolite 4-chloro-18-nor-17β-hydroxymethyl-17α-methyl-5β-androst-13-en-3α-ol (M3). LC-HRMSMS, GC-MSMS, fractionation and derivatization experiments are combined to identify and characterize for the first time two different glucuronide-acid conjugates of this metabolite in positive human urine samples. In addition, a third glucuronide metabolite was identified, however without isomeric structure determination. The detection of these metabolites is particularly interesting for confirmation analyses, as the method is rapid and requires little sample material.
Forlag
Elsevier
Sitering
Göschl L, Gmeiner G, Gärtner P, Steinacher, Forsdahl G. Detection of DHCMT long-term metabolite glucuronides with LC-MSMS as an alternative approach to conventional GC-MSMS analysis. Steroids. 2022;180
Metadata
Vis full innførsel
Samlinger
  • Artikler, rapporter og annet (farmasi) [393]
Copyright 2022 The Author(s)

Bla

Bla i hele MuninEnheter og samlingerForfatterlisteTittelDatoBla i denne samlingenForfatterlisteTittelDato
Logg inn

Statistikk

Antall visninger
UiT

Munin bygger på DSpace

UiT Norges Arktiske Universitet
Universitetsbiblioteket
uit.no/ub - munin@ub.uit.no

Tilgjengelighetserklæring