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dc.contributor.authorMcClusky, Leon Mendel
dc.date.accessioned2022-08-15T07:17:20Z
dc.date.available2022-08-15T07:17:20Z
dc.date.issued2022-06-07
dc.description.abstractThe process of spermatogenesis features signifcant germ cell loss through apoptosis. Routine histology of the testes of wellstudied animal models hardly discloses any trace of their phagocytic clearance by the supporting Sertoli cells. This review highlights lessons learnt from the cystic, diametric testes of some seasonally migrating elasmobranchs (e.g., spiny dogfsh and blue sharks) that ofer unconventional investigative paradigms to study these phenomena as these organs readily disclose a pronounced apoptosis gradient afecting exclusively spermatogonial clones that each are enclosed with their own Sertoli cells in spherical structures called spermatocysts. This gradient is visible at a certain time of year in the spermatogenically active shark, and peaks in mature spermatogonial cysts as clustered deaths with sporadic, and not massive secondary necrosis. Conversely, immature spermatogonial cysts in blue sharks reveal a characteristic periluminal display of single apoptotic deaths. Tracing aberrations in the immunostaining patterns of the conserved cell cycle marker, proliferating cell nuclear antigen, the gradual progression of the death process in individual or coalesced spermatogonia in contiguous cysts becomes clear. The multiple apoptotic nuclear fragmentation morphologies inform also of a protracted death process involving three diferent morphological routes of nuclear fragmentation (of which some are TUNEL-positive and other TUNEL-negative) and concomitant chromatin compaction that culminate in freed apoptotic bodies (i.e., secondary necrosis). It is discussed that the staggered spermatogonial deaths and accompanying intermittent secondary necrosis in mature blue shark spermatogonial cysts may well relate to the low phagocytosis capacity of cyst’s Sertoli cells that are still functionally naïve.en_US
dc.identifier.citationMcClusky L. Several routes of cell death to secondary necrosis in the elasmobranch testis. Apoptosis. 2022;27(7-8):454-464en_US
dc.identifier.cristinIDFRIDAID 2029344
dc.identifier.doi10.1007/s10495-022-01733-0
dc.identifier.issn1360-8185
dc.identifier.issn1573-675X
dc.identifier.urihttps://hdl.handle.net/10037/26164
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.journalApoptosis
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleSeveral routes of cell death to secondary necrosis in the elasmobranch testisen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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