dc.contributor.author | Diaz Cánova, Diana Karina | |
dc.contributor.author | Moens, Ugo | |
dc.contributor.author | Brinkmann, Annika | |
dc.contributor.author | Nitsche, Andreas | |
dc.contributor.author | Okeke, malachy ifeanyi | |
dc.date.accessioned | 2022-11-14T12:33:48Z | |
dc.date.available | 2022-11-14T12:33:48Z | |
dc.date.issued | 2022-05-03 | |
dc.description.abstract | Orthopoxviruses (OPXVs) not only infect their natural hosts, but some OPXVs can also
cause disease in humans. Previously, we partially characterized an OPXV isolated from
an 18-year-old male living in Northern Norway. Restriction enzyme analysis and partial
genome sequencing characterized this virus as an atypical cowpox virus (CPXV), which
we named CPXV-No-H2. In this study, we determined the complete genome sequence
of CPXV-No-H2 using Illumina and Nanopore sequencing. Our results showed that the
whole CPXV-No-H2 genome is 220,276 base pairs (bp) in length, with inverted terminal
repeat regions of approximately 7 kbp, containing 217 predicted genes. Seventeen
predicted CPXV-No-H2 proteins were most similar to OPXV proteins from the Old World,
including Ectromelia virus (ECTV) and Vaccinia virus, and North America, Alaskapox virus
(AKPV). CPXV-No-H2 has a mosaic genome with genes most similar to other OPXV
genes, and seven potential recombination events were identified. The phylogenetic
analysis showed that CPXV-No-H2 formed a separate clade with the German CPXV
isolates CPXV_GerMygEK938_17 and CPXV_Ger2010_MKY, sharing 96.4 and 96.3%
nucleotide identity, respectively, and this clade clustered closely with the ECTV-OPXV
Abatino clade. CPXV-No-H2 is a mosaic virus that may have arisen out of several
recombination events between OPXVs, and its phylogenetic clustering suggests that
ECTV-Abatino-like cowpox viruses form a distinct, new clade of cowpox viruses. | en_US |
dc.identifier.citation | Diaz Cánova, Moens, Brinkmann, Nitsche, Okeke. Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus. Frontiers in Microbiology. 2022;13 | en_US |
dc.identifier.cristinID | FRIDAID 2045889 | |
dc.identifier.doi | 10.3389/fmicb.2022.868887 | |
dc.identifier.issn | 1664-302X | |
dc.identifier.uri | https://hdl.handle.net/10037/27360 | |
dc.language.iso | eng | en_US |
dc.publisher | Frontiers Media | en_US |
dc.relation.ispartof | Diaz-Cánova, D. (2023). Evolutionary genomics of cowpox virus and recombination in vitro between a naturally occurring cowpox virus and a vaccinia virus vectored influenza vaccine. (Doctoral thesis). <a href=https://hdl.handle.net/10037/29180>https://hdl.handle.net/10037/29180</a>. | |
dc.relation.journal | Frontiers in Microbiology | |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2022 The Author(s) | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | en_US |
dc.rights | Attribution 4.0 International (CC BY 4.0) | en_US |
dc.title | Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus | en_US |
dc.type.version | publishedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |