Cooling to hypothermic circulatory arrest by immersion vs. cardiopulmonary bypass (CPB): Worse outcome after rewarming in immersion cooled pigs

Abstract

Introduction: Cooling by cardiopulmonary bypass (CPB) to deep hypothermic cardiac arrest (HCA) for cardiac surgical interventions, followed by CPB-rewarming is performed on a routine basis with relatively low mortality. In contrast, victims of deep accidental hypothermia rewarmed with CPB generally have a much worse prognosis. Thus, we have developed an intact pig model to compare effects on perfusion pressures and global oxygen delivery (DO₂) during immersion cooling versus cooling by CPB. Further, we compared the effects of CPB-rewarming between groups, to restitute cardiovascular function, brain blood flow, and brain metabolism.<p> <p>Materials and Methods: Total sixteen healthy, anesthetized juvenile (2–3 months) castrated male pigs were randomized in a prospective, open placebo-controlled experimental study to immersion cooling (IMM_c, n = 8), or cooling by CPB (CPB_c, n = 8). After 75 minutes of deep HCA in both groups, pigs were rewarmed by CPB. After weaning from CPB surviving animals were observed for 2 h before euthanasia. <p>Results: Survival rates at 2 h after completed rewarming were 4 out of 8 in the IMM_c group, and 8 out of 8 in the CPB_c group. Compared with the CPB_c-group, IMM_c animals showed significant reduction in DO₂, mean arterial pressure (MAP), cerebral perfusion pressure, and blood flow during cooling below 25◦C as well as after weaning from CPB after rewarming. After rewarming, brain blood flow returned to control in CPB_c animals only, and brain micro dialysate-data showed a significantly increase in the lactate/pyruvate ratio in IMM_c vs. CPB_c animals <p>Conclusion: Our data indicate that, although global O₂ consumption was independent of DO₂, regional ischemic damage may have taken place during cooling in the brain of IMM_c animals below 25◦C. The need for prolonged extracorporeal membrane oxygenation (ECMO) should be considered in all victims of accidental hypothermic arrest that cannot be weaned from CPB immediately after rewarming.