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dc.contributor.authorFoell, Dirk
dc.contributor.authorSaers, Melanie
dc.contributor.authorPark, Carolin
dc.contributor.authorBrix, Ninna
dc.contributor.authorGlerup, Mia
dc.contributor.authorKessel, Christoph
dc.contributor.authorWittkowski, Helmut
dc.contributor.authorHinze, Claas
dc.contributor.authorBerntson, Lillemor
dc.contributor.authorFasth, Anders
dc.contributor.authorMyrup, Charlotte
dc.contributor.authorNordal, Ellen Berit
dc.contributor.authorRygg, Marite
dc.contributor.authorHasle, Henrik
dc.contributor.authorAlbertsen, Birgitte Klug
dc.contributor.authorHerlin, Troels
dc.contributor.authorHolzinger, Dirk
dc.contributor.authorNiederberger, Christian
dc.contributor.authorSchlüter, Bernhard
dc.date.accessioned2024-01-12T12:53:38Z
dc.date.available2024-01-12T12:53:38Z
dc.date.issued2023-10-25
dc.description.abstractBackground Differential diagnosis in children with signs of unprovoked inflammation can be challenging. In particular, differentiating systemic juvenile idiopathic arthritis (SJIA) from other diagnoses is difficult. We have recently validated the complex of myeloid-related proteins 8/14 (MRP8/14, also known as S100A8/A9 complex or serum calprotectin) as a helpful biomarker supporting the diagnosis of SJIA. The results were subsequently confirmed with a commercial ELISA. However, further optimization of the analytical technology is important to ensure its feasibility for large-scale use in routine laboratory settings.<p> <p>Methods To evaluate the accuracy in identifying children with SJIA, the performance of a particle-enhanced immuno-turbidimetric assay for serum calprotectin (sCAL turbo) on an automated laboratory instrument was analyzed. Samples from 615 children were available with the diagnoses SJIA (n=99), non-systemic JIA (n=169), infections (n=51), other infammatory diseases (n=126), and acute lymphoblastic leukemia (ALL, n=147). In addition, samples from 23 healthy controls were included. <p>Results The sCAL turbo assay correlated well with the MRP8/14 ELISA used in previous validation studies (r=0.99, p<0.001). It could reliably differentiate SJIA from all other diagnoses with signifcant accuracy (cutof at 10,500 ng/ml, sensitivity 84%, specifcity 94%, ROC area under curve 0.960, p<0.001). <p>Conclusions Serum calprotectin analyses are a helpful tool supporting the diagnosis of SJIA in children with prolonged fever or inflammatory disease. Here, we show that an immuno-turbidimetric assay for detection of serum calprotectin on an automated laboratory instrument can be implemented in clinical laboratory settings to facilitate its use as a diagnostic routine test in clinical practice.en_US
dc.identifier.citationFoell, Saers, Park, Brix, Glerup, Kessel, Wittkowski, Hinze, Berntson, Fasth, Myrup, Nordal, Rygg, Hasle, Albertsen, Herlin, Holzinger, Niederberger, Schlüter. A novel serum calprotectin (MRP8/14) particle-enhanced immuno-turbidimetric assay (sCAL turbo) helps to differentiate systemic juvenile idiopathic arthritis from other diseases in routine clinical laboratory settings. Molecular and cellular pediatrics. 2023;10(1)en_US
dc.identifier.cristinIDFRIDAID 2196931
dc.identifier.doi10.1186/s40348-023-00168-0
dc.identifier.issn2194-7791
dc.identifier.urihttps://hdl.handle.net/10037/32452
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalMolecular and cellular pediatrics
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleA novel serum calprotectin (MRP8/14) particle-enhanced immuno-turbidimetric assay (sCAL turbo) helps to differentiate systemic juvenile idiopathic arthritis from other diseases in routine clinical laboratory settingsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)