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dc.contributor.authorDe Barba, Marta
dc.contributor.authorBaur, Molly
dc.contributor.authorBoyer, Frédéric
dc.contributor.authorFumagalli, Luca
dc.contributor.authorKonec, Marjeta
dc.contributor.authorMiquel, Christian
dc.contributor.authorPazhenkova, Elena
dc.contributor.authorRemollino, Nadège
dc.contributor.authorSkrbinšek, Tomaž
dc.contributor.authorStoffel, Céline
dc.contributor.authorTaberlet, Pierre Robert Michel
dc.description.abstractContinued advancements in environmental DNA (eDNA) research have made it possible to access intraspecific variation from eDNA samples, opening new opportunities to expand non-invasive genetic studies of wildlife populations. However, the use of eDNA samples for individual genotyping, as typically performed in non-invasive genetics, still remains elusive. We present successful individual genotyping of eDNA obtained from snow tracks of three large carnivores: brown bear (Ursus arctos), European lynx (Lynx lynx) and wolf (Canis lupus). DNA was extracted using a protocol for isolating water eDNA and genotyped using amplicon sequencing of short tandem repeats (STR), and for brown bear a sex marker, on a high-throughput sequencing platform. Individual genotypes were obtained for all species, but genotyping performance differed among samples and species. The proportion of samples genotyped to individuals was higher for brown bear (5/7) and wolf (7/10) than for lynx (4/9), and locus genotyping success was greater for brown bear (0.88). The sex marker was typed in six out of seven brown bear samples. Results for three species show that reliable individual genotyping, including sex identification, is now possible from eDNA in snow tracks, underlining its vast potential to complement the non-invasive genetic methods used for wildlife. To fully leverage the application of snow track eDNA, improved understanding of the ideal species- and site-specific sampling conditions, as well as laboratory methods promoting genotyping success, is needed. This will also inform efforts to retrieve and type nuclear DNA from other eDNA samples, thereby advancing eDNA-based individual and population-level studies.en_US
dc.identifier.citationDe Barba, Baur, Boyer, Fumagalli, Konec, Miquel, Pazhenkova, Remollino, Skrbinšek, Stoffel, Taberlet. Individual genotypes from environmental DNA: Fingerprinting snow tracks of three large carnivore species. Molecular Ecology Resources. 2023en_US
dc.identifier.cristinIDFRIDAID 2219580
dc.relation.journalMolecular Ecology Resources
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleIndividual genotypes from environmental DNA: Fingerprinting snow tracks of three large carnivore speciesen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US

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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)