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dc.contributor.authorChan, Dennis Tin Chat
dc.contributor.authorBernstein, Hans Christopher
dc.contributor.authorBaldwin, Geoff S.
dc.description.abstractBroad-host-range synthetic biology is an emerging frontier that aims to expand our current engineerable domain of microbial hosts for biodesign applications. As more novel species are brought to “model status,” synthetic biologists are discovering that identically engineered genetic circuits can exhibit different performances depending on the organism it operates within, an observation referred to as the “chassis effect.” It remains a major challenge to uncover which genome-encoded and biological determinants will underpin chassis effects that govern the performance of engineered genetic devices. In this study, we compared model and novel bacterial hosts to ask whether phylogenomic relatedness or similarity in host physiology is a better predictor of genetic circuit performance. This was accomplished using a comparative framework based on multivariate statistical approaches to systematically demonstrate the chassis effect and characterize the performance dynamics of a genetic inverter circuit operating within 6 Gammaproteobacteria. Our results solidify the notion that genetic devices are strongly impacted by the host context. Furthermore, we formally determined that hosts exhibiting more similar metrics of growth and molecular physiology also exhibit more similar performance of the genetic inverter, indicating that specific bacterial physiology underpins measurable chassis effects. The result of this study contributes to the field of broad-host-range synthetic biology by lending increased predictive power to the implementation of genetic devices in less-established microbial hosts.en_US
dc.identifier.citationChan, Baldwin, Bernstein. Revealing the Host-Dependent Nature of an Engineered Genetic Inverter in Concordance with Physiology. BioDesign Research. 2023;5en_US
dc.identifier.cristinIDFRIDAID 2193360
dc.publisherAmerican Association for the Advancement of Scienceen_US
dc.relation.journalBioDesign Research
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleRevealing the Host-Dependent Nature of an Engineered Genetic Inverter in Concordance with Physiologyen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US

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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)