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Adjusting the Fitting of Fluorescence-Based Dose-Response Kinase Inhibition Assay to Account for Fluorescent Inhibitors

Permanent link
https://hdl.handle.net/10037/33059
DOI
https://doi.org/10.1089/adt.2023.044
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Date
2023-10-11
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Author
Petit, Guillaume Axel
Abstract
Fluorescence is routinely used to monitor kinase inhibition in commercial assays. Occasionally fluorescent compounds can interfere with the fluorescent reading. To address this issue, the problematic data are usually truncated to improve the fit, however, this approach raises ethical and reproducibility concerns. Instead, it is suggested to adjust the fitting formula, to account for the autofluorescence of the compounds and improve the fit of the data compared with a naive approach. Finally, it was noticed that truncating the data can result in a small underestimation of the IC50 values and should therefore be used carefully.
Publisher
Mary Ann Liebert
Citation
Petit. Adjusting the Fitting of Fluorescence-Based Dose-Response Kinase Inhibition Assay to Account for Fluorescent Inhibitors. Assay and drug development technologies. 2023;21(7)
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  • Artikler, rapporter og annet (Norges fiskerihøgskole) [1053]
Copyright 2023 The Author(s)

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