Show simple item record

dc.contributor.authorFenton, Christopher Graham
dc.contributor.authorRay, Mithlesh Kumar
dc.contributor.authorPaulssen, Ruth Erika Hildegard Hracky
dc.date.accessioned2024-05-30T07:09:27Z
dc.date.available2024-05-30T07:09:27Z
dc.date.issued2024-04-05
dc.description.abstractThe study aimed to identify common differentially expressed lncRNAs from manually curated ulcerative colitis (UC) gene expression omnibus (GEO) datasets. Nine UC transcriptomic datasets of clearly annotated human colonic biopsies were included in the study. The datasets were manually curated to select active UC samples and controls. R packages geneknitR, gprofiler, clusterProfiler were used for gene symbol annotation. The R EdgeR package was used to analyze differential expression. This resulted in a total of nineteen lncRNAs that were differentially expressed in at least three datasets of the nine GEO datasets. Several of the differentially expressed lncRNAs found in UC were associated with promoting colorectal cancer (CRC) through regulating gene expression, epithelial to mesenchymal transition (EMT), cell cycle progression, and by promoting tumor proliferation, invasion, and migration. The expression of several lncRNAs varied between disease states and tissue locations within the same disease state. The identified differentially expressed lncRNAs may function as general markers for active UC independent of biopsy location, age, gender, or treatment, thereby representing a comparative resource for future comparisons using available GEO UC datasets.en_US
dc.identifier.citationFenton CG, Ray MK, Paulssen RH. Challenges in Defining a Reference Set of Differentially Expressed lncRNAs in Ulcerative Colitis by Meta-Analysis. Current Issues in Molecular Biology. 2024;46(4):3164-3174en_US
dc.identifier.cristinIDFRIDAID 2259476
dc.identifier.doi10.3390/cimb46040198
dc.identifier.issn1467-3037
dc.identifier.issn1467-3045
dc.identifier.urihttps://hdl.handle.net/10037/33646
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.ispartofRay, M.R. (2024). Long non-coding RNAs in ulcerative colitis. (Doctoral thesis). <a href=https://hdl.handle.net/10037/33648>https://hdl.handle.net/10037/33648</a>.
dc.relation.journalCurrent Issues in Molecular Biology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleChallenges in Defining a Reference Set of Differentially Expressed lncRNAs in Ulcerative Colitis by Meta-Analysisen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record

Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)