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dc.contributor.advisorStuge, Tor Brynjar
dc.contributor.advisorHusebekk, Anne
dc.contributor.authorKillie, Ida Løken
dc.date.accessioned2011-06-09T07:36:38Z
dc.date.available2011-06-09T07:36:38Z
dc.date.issued2010-11-15
dc.description.abstractNeonatal alloimmune thrombocytopenia (NAIT) is most commonly caused by destruction of foetal platelets by maternal antibodies reactive to human platelet antigen (HPA)-1a. The activation of antigen-primed B cells to differentiate to antibody-secreting plasma cells usually requires help from CD4 T cells. The strong association between anti-HPA-1a-production and the MHC allele HLA-DRB3*0101 supports that this notion is also valid in the context of NAIT, and suggests the activation of HPA-1a-specific T cells as the most critical event of immunization. In this study, an improved protocol for enrichment, identification and efficient isolation of HPA-1a-specific CD4 T cells is presented. By replacing foetal bovine serum with human serum, enrichment of antigen-specific CD4 T cells improved dramatically. Identification and isolation of HPA-1a-specific CD4 T cells greatly improved when combining the CFSE proliferation assay with a second stimulation with antigen and subsequent assay for surface detection of TNF production. HPA-1a-specific CD4 T cells could also be identified in the CFSE proliferation assay as proliferating T cells with down-regulated expression of CD4. HPA-1a-specific T cells isolated from immunized women may serve as useful tools for investigating the cellular immune response to HPA-1a, and for developing strategies to prevent immunization in HPA-incompatible pregnancies, e.g. through TCR epitope mapping and examinations of the immunogenicity of the HPA-1a antigen at the amino-acid level.en
dc.identifier.urihttps://hdl.handle.net/10037/3389
dc.identifier.urnURN:NBN:no-uit_munin_3112
dc.language.isoengen
dc.publisherUniversitetet i Tromsøen
dc.publisherUniversity of Tromsøen
dc.rights.accessRightsopenAccess
dc.rights.holderCopyright 2010 The Author(s)
dc.subject.courseIDMBI-3910en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716en
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical immunology: 716en
dc.subjectNeonatal alloimmun trombocytopenien
dc.subjectNeonatal alloimmune thrombocytopeniaen
dc.subjectantigen-specific T cellsen
dc.subjectantigen-spesifikke T celleren
dc.subjectNAITen
dc.subjectFNAITen
dc.subjectNAITPen
dc.subjectalloimmunityen
dc.titleDevelopment of new tissue culture protocols for enrichment of CD4 T cells associated with neonatal alloimmune thrombocytopeniaen
dc.typeMaster thesisen
dc.typeMastergradsoppgaveen


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