Institutt for medisinsk biologi: Recent submissions
Now showing items 241-260 of 1154
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Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-28)Liver Sinusoidal Endothelial Cells (LSEC) line the hepatic vasculature providing blood filtration via transmembrane nanopores called fenestrations. These structures are 50−300 nm in diameter, which is below the resolution limit of a conventional light microscopy. To date, there is no standardized method of fenestration image analysis. With this study, we provide and compare three different approaches: ... -
Interplay of immune and kidney resident cells in the formation of tertiary lymphoid structures in lupus nephritis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-10-27)Kidney involvement confers significant morbidity and mortality in patients with systemic lupus erythematosus (SLE). The pathogenesis of lupus nephritis (LN) involves diverse mechanisms instigated by elements of the autoimmune response which alter the biology of kidney resident cells. Processes in the glomeruli and in the interstitium may proceed independently albeit crosstalk between the two is ... -
Novel human melanoma brain metastasis models in athymic nude fox1nu mice: Site-specific metastasis patterns reflecting their clinical origin
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-10-06)Background: Malignant melanomas frequently metastasize to the brain, but metastases in the cerebellum are underrepresented compared with metastases in the cerebrum.<p> <p>Methods: We established animal models by injecting intracardially in athymic nude fox1<sup>nu</sup> mice two human melanoma cell lines, originating from a cerebral metastasis (HM19) and a cerebellar metastasis (HM86).<p> <p>Results: ... -
Autofluorescence in freshly isolated adult human liver sinusoidal cells
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-13)Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment ... -
Inferring early-life host and microbiome functions by mass spectrometry-based metaproteomics and metabolomics
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-20)Humans have a long-standing coexistence with microorganisms. In particular, the microbial community that populates the human gastrointestinal tract has emerged as a critical player in governing human health and disease. DNA and RNA sequencing techniques that map taxonomical composition and genomic potential of the gut community have become invaluable for microbiome research. However, deriving a ... -
Impact of KRAS, BRAF and microsatellite instability status after cytoreductive surgery and HIPEC in a national cohort of colorectal peritoneal metastasis patients
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-09)Background - Patients with metastatic colorectal cancer (mCRC) carrying BRAF (mutBRAF) or KRAS mutation (mutKRAS) have an inferior prognosis after liver or lung surgery, whereas the prognostic role in the context of peritoneal metastasis (PM) after cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) has been less investigated.<p> <p>Methods - In total, 257 patients ... -
Heart Angiotensin- Converting Enzyme and Angiotensin-Converting Enzyme 2 Gene Expression Associated With Male Sex and Salt-Sensitive Hypertension in the Dahl Rat.
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-19)Angiotensin-converting enzyme 2 (ACE 2) in the heart including its sex dependency in the hypertensive heart, has not been much studied compared to ACE. In the present study, we used the Dahl salt-sensitive rat exposed to fructose and salt to model a hypertensive phenotype in males, females, and ovariectomized females. Blood pressure was measured by the tale-cuff technique in the conscious state. ... -
Occupational Allergic Sensitization Among Workers Processing King Crab (Paralithodes camtschaticus) and Edible Crab (Cancer pagurus) in Norway and Identification of Novel Putative Allergenic Proteins
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-23)Introduction: Asthma and allergy occur frequently among seafood processing workers, with the highest prevalence seen in the crustacean processing industry. In this study we established for the first time the prevalence of allergic sensitization in the Norwegian king- and edible crab processing industry and characterized the IgE-reactive proteins.<p> <p>Materials and Methods: Two populations of ... -
Fetal/neonatal alloimmune thrombocytopenia: A systematic review of impact of HLA-DRB3∗01:01 on fetal/neonatal outcome
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-07-27)The most common, severe cases of fetal and neonatal alloimmune thrombocytopenia among whites are caused by antibodies against human platelet antigen 1a (HPA-1a). The aims of this systematic review and meta-analysis are to determine the association between maternal HLA-DRB3*01:01 and: (1) HPA-1a-alloimmunization and (2) neonatal outcome in children born of HPA-1a-immunized women. A systematic ... -
Persistent pulmonary pathology after COVID-19 is associated with high viral load, weak antibody response, and high levels of matrix metalloproteinase-9
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-01)The association between pulmonary sequelae and markers of disease severity, as well as pro-fibrotic mediators, were studied in 108 patients 3 months after hospital admission for COVID-19. The COPD assessment test (CAT-score), spirometry, diffusion capacity of the lungs (DL<sub>CO</sub>), and chest-CT were performed at 23 Norwegian hospitals included in the NOR-SOLIDARITY trial, an open-labelled, ... -
Endoscopic full-thickness dissection (EFTD) in the rectum: a case series
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-28)Background Rectal endoscopic full- thickness dissection (EFTD) using a fexible colonoscope is an alternative to the wellestablished trans-anal endoscopic microsurgery (TEM) and the trans-anal minimally invasive surgery (TAMIS) techniques for resecting dysplastic or malignant rectal lesions. This study evaluated EFTD safety by analyzing outcomes of the frst patients to undergo rectal EFTD at the ... -
Heart Angiotensin-Converting Enzyme and Angiotensin-Converting Enzyme 2 Gene Expression Associated With Male Sex and Salt-Sensitive Hypertension in the Dahl Rat
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-19)Angiotensin-converting enzyme 2 (ACE 2) in the heart including its sex dependency in the hypertensive heart, has not been much studied compared to ACE. In the present study, we used the Dahl salt-sensitive rat exposed to fructose and salt to model a hypertensive phenotype in males, females, and ovariectomized females. Blood pressure was measured by the tale-cuff technique in the conscious state. ... -
Impact of maturation on myocardial response to ischemia and the effectiveness of remote preconditioning in male rats
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-10-12)Aging attenuates cardiac tolerance to ischemia/reperfusion (I/R) associated with defects in protective cell signaling, however, the onset of this phenotype has not been completely investigated. This study aimed to compare changes in response to I/R and the effects of remote ischemic preconditioning (RIPC) in the hearts of younger adult (3 months) and mature adult (6 months) male Wistar rats, with ... -
Covid-19 transmission in fitness centers in Norway - a randomized trial
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-16)Background: Closed fitness centers during the Covid-19 pandemic may negatively impact health and wellbeing. We assessed whether training at fitness centers increases the risk of SARS-CoV-2 virus infection.<p> <p>Methods: In a two-group parallel randomized controlled trial, fitness center members aged 18 to 64 without Covid-19-relevant comorbidities, were randomized to access to training at a ... -
Bacterial genomic epidemiology with mixed samples
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-15)Genomic epidemiology is a tool for tracing transmission of pathogens based on whole-genome sequencing. We introduce the mGEMS pipeline for genomic epidemiology with plate sweeps representing mixed samples of a target pathogen, opening the possibility to sequence all colonies on selective plates with a single DNA extraction and sequencing step. The pipeline includes the novel mGEMS read binner for ... -
Vaccine Induced Immune Thrombotic Thrombocytopenia Causing a Severe Form of Cerebral Venous Thrombosis With High Fatality Rate: A Case Series
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-30)During a 2-week period, we have encountered five cases presenting with the combination of cerebral venous thrombosis (CVT), intracerebral hemorrhage and thrombocytopenia. A clinical hallmark was the rapid and severe progression of disease in spite of maximum treatment efforts, resulting in fatal outcome in for 4 out of 5 patients. All cases had received ChAdOx1 nCov-19 vaccine 1–2 weeks earlier and ... -
Zinc-Chelating Compounds as Inhibitors of Human and Bacterial Zinc Metalloproteases
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-22)Inhibition of bacterial virulence is believed to be a new treatment option for bacterial infections. In the present study, we tested dipicolylamine (DPA), tripicolylamine (TPA), tris pyridine ethylene diamine (TPED), pyridine and thiophene derivatives as putative inhibitors of the bacterial virulence factors thermolysin (TLN), pseudolysin (PLN) and aureolysin (ALN) and the human zinc metalloproteases, ... -
Comparative Genomics Reveals Factors Associated with Phenotypic Expression of Wolbachia
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-05-18)Wolbachia is a widespread, vertically transmitted bacterial endosymbiont known for manipulating arthropod reproduction. Its most common form of reproductive manipulation is cytoplasmic incompatibility (CI), observed when a modification in the male sperm leads to embryonic lethality unless a compatible rescue factor is present in the female egg. CI attracts scientific attention due to its implications ... -
Risk of a permanent work-related disability pension after incident venous thromboembolism in Denmark: A population-based cohort study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-31)Background - Long-term complications of venous thromboembolism (VTE) hamper physical function and impair quality of life; still, it remains unclear whether VTE is associated with risk of permanent work-related disability. We aimed to assess the association between VTE and the risk of receiving a permanent work-related disability pension and to assess whether this association was explained by ... -
A Screening for Virus Infections in Eight Herds of Semi-domesticated Eurasian Tundra Reindeer (Rangifer tarandus tarandus) in Norway, 2013–2018
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-10-12)Background: Previous serological screenings have indicated that Eurasian semi-domesticated tundra reindeer (Rangifer tarandus tarandus) in Finnmark, Northern Norway, are exposed to alphaherpesvirus, gammaherpesvirus and pestivirus. Alphaherpesvirus (i.e., Cervid herpesvirus 2; CvHV2) has been identified as the transmissible component of infectious keratoconjunctivitis (IKC). Limited knowledge exists ...