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dc.contributor.authorKashobwe, Lackson
dc.contributor.authorSadrabadi, Faezeh
dc.contributor.authorBrunken, Lars
dc.contributor.authorMiranda Fernandes Coelho, Ana Carolina
dc.contributor.authorSandanger, Torkjel Manning
dc.contributor.authorBraeuning, Albert
dc.contributor.authorBuhrke, Thorsten
dc.contributor.authorÖberg, Mattias
dc.contributor.authorHamers, Timo
dc.contributor.authorLeonards, Pim.E.G.
dc.date.accessioned2024-10-04T08:27:25Z
dc.date.available2024-10-04T08:27:25Z
dc.date.issued2024-06-10
dc.description.abstractPer- and polyfluoroalkyl substances (PFAS) are synthetic chemicals used in various industrial and consumer products. They have gained attention due to their ubiquitous occurrence in the environment and potential for adverse effects on human health, often linked to immune suppression, hepatotoxicity, and altered cholesterol metabolism. This study aimed to explore the impact of ten individual PFAS, 3 H-perfluoro-3-[(3-methoxypropoxy) propanoic acid] (PMPP/Adona), ammonium perfluoro-(2-methyl-3-oxahexanoate) (HFPO-DA/GenX), perfluorobutanoic acid (PFBA), perfluorobutanesulfonic acid (PFBS), perfluorodecanoic acid (PFDA), perfluorohexanoic acid (PFHxA), perfluorohexanesulfonate (PFHxS), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA), and perfluorooctanesulfonic acid (PFOS) on the lipid metabolism in human hepatocyte-like cells (HepaRG). These cells were exposed to different concentrations of PFAS ranging from 10 µM to 5000 µM. Lipids were extracted and analyzed using liquid chromatography coupled with mass spectrometry (LC- MS-QTOF). PFOS at 10 µM and PFOA at 25 µM increased the levels of ceramide (Cer), diacylglycerol (DAG), N-acylethanolamine (NAE), phosphatidylcholine (PC), and triacylglycerol (TAG) lipids, while PMPP/Adona, HFPO-DA/GenX, PFBA, PFBS, PFHxA, and PFHxS decreased the levels of these lipids. Furthermore, PFOA and PFOS markedly reduced the levels of palmitic acid (FA 16.0). The present study shows distinct concentration-dependent effects of PFAS on various lipid species, shedding light on the implications of PFAS for essential cellular functions. Our study revealed that the investigated legacy PFAS (PFOS, PFOA, PFBA, PFDA, PFHxA, PFHxS, and PFNA) and alternative PFAS (PMPP/Adona, HFPO-DA/GenX and PFBS) can potentially disrupt lipid homeostasis and metabolism in hepatic cells. This research offers a comprehensive insight into the impacts of legacy and alternative PFAS on lipid composition in HepaRG cells.en_US
dc.identifier.citationKashobwe, Sadrabadi, Brunken, Miranda Fernandes Coelho, Sandanger, Braeuning, Buhrke, Öberg, Hamers, Leonards. Legacy and alternative per- and polyfluoroalkyl substances (PFAS) alter the lipid profile of HepaRG cells. Toxicology. 2024;506en_US
dc.identifier.cristinIDFRIDAID 2283134
dc.identifier.doi10.1016/j.tox.2024.153862
dc.identifier.issn0300-483X
dc.identifier.issn1879-3185
dc.identifier.urihttps://hdl.handle.net/10037/35044
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalToxicology
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/ 860665/EU/Innovative Training Network on PER and polyfluorinated alkyl substances towards the Future Of Research and its Communication in Europe 3/PERFORCE3/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleLegacy and alternative per- and polyfluoroalkyl substances (PFAS) alter the lipid profile of HepaRG cellsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)