Inhibition of Tooth Demineralization by CPP-ACP and NaF: An In Vitro study with MicroCT

Abstract

<p>Aim: The study looked to determine whether casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) differed from toothpastes containing NaF in its ability to inhibit demineralization. <p>Materials: Extracted permanent molars were sectioned and allocated to 4 different groups. the pieces were treated 30 minutes daily for 7 days by the following agents: 1450 ppm NaF, 5000ppm NaF, CPP-ACP, CPP-ACPF (900ppm NaF). The pieces were scanned with MicroCT after treatment, then submerged in an acid-buffer solution (pH 4.4) for 24h, 72h and 120h. The pieces were scanned after each time point. Total mineral change (TMC) and Enamel mineral change (EMC) were calculated from each period from the MicroCT scans. <p>Results: TMC after 24h was significantly greater in CPP-ACPF than all other groups (p<0.05, one-way ANOVA with Bonferroni comparison) TMC after 72h was significantly greater in CPP-ACPF compared to 1450ppm NaF and 5000ppm NaF, and greater in CPP-ACP compared to 5000 ppm NaF. The same was true for TMC after 120h as TMC after 72h. There was no significant difference between groups in EMC after 24h (p>0.05). EMC after 72h was significantly greater in CPP-ACP compared to 5000ppm, and CPP-ACPF compared to all other groups. The same was true for EMC after 120h as EMC after 72h. <p>Conclusions: we found that CPP-ACP did not significantly differ in performance from NaF at 1450 ppm but performed worse than NaF at 5000ppm. Differences between groups were generally bigger in TMC than EMC. We conclude that CPP-ACP may be used as an adjunct to toothpastes with NaF but not as a replacement.