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dc.contributor.advisorRavna, Aina
dc.contributor.advisorSager, Georg
dc.contributor.advisorLyså, Roy
dc.contributor.authorØrvoll, Elin
dc.date.accessioned2013-04-30T10:53:10Z
dc.date.available2013-04-30T10:53:10Z
dc.date.issued2011-06-24
dc.description.abstractABCC5 is a member of the superfamily of ABC-transporters, and it has been identified as an efflux transporter of cGMP. This transporter is also involved in export of antibiotic and cytostatic drugs from target cells, and as such represents a challenge in treatment of cancer and infectious diseases. In order to find inhibitors to ABCC5 mediated drug efflux, compounds predicted as potent inhibitors by virtual ligand screening (VLS) were chosen for in-vitro studies by the use of human erythrocyte inside-out vesicles (IOV). The procedure for IOV preparation was improved, and transport assays were performed where the inhibiting effects of the various compounds on transport of cGMP into inside-out vesicles were measured. Several of these compounds showed a potent inhibiting effect on cGMP transport, and the few that were chosen for further characterization showed more potent inhibition of ABCC5 than the known ABCC5 and PDE5 inhibitor sildenafil.en
dc.identifier.urihttps://hdl.handle.net/10037/5137
dc.identifier.urnURN:NBN:no-uit_munin_4850
dc.language.isoengen
dc.publisherUniversitetet i Tromsøen
dc.publisherUniversity of Tromsøen
dc.rights.accessRightsopenAccess
dc.rights.holderCopyright 2011 The Author(s)
dc.subject.courseIDMBI-3910en
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750en
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en
dc.titlePartial characterization of predicted ABCC5 inhibitors by the aid of human erythrocyte inside-out vesiclesen
dc.typeMaster thesisen
dc.typeMastergradsoppgaveen


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