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dc.contributor.authorIliev, Dimitri Borisov
dc.contributor.authorHansen, Tom Egil
dc.contributor.authorJørgensen, Sven Martin
dc.contributor.authorKrasnov, Aleksei
dc.contributor.authorJørgensen, Jorunn B
dc.date.accessioned2014-02-27T12:48:34Z
dc.date.available2014-02-27T12:48:34Z
dc.date.issued2013
dc.description.abstractThe Mitogen-activated protein kinases (MAPK) are involved in transmitting intracellular signals downstream of diverse cell surface receptors and mediate the response to ligands such as growth factors, hormones and cytokines. In addition, MAPK are critically involved in the innate immune response to pathogen-derived substances, commonly referred to as pathogen-associated molecular patterns (PAMPs), such as bacterial lipopolysaccharide (LPS) and bacterial DNA rich in CpG dinucleotides. Currently, a great deal of knowledge is available about the involvement of MAPK in the innate immune response to PAMPs in mammals; however, little is known about the role of the different MAPK classes in the immune response to PAMPs in lower vertebrates. In the current study, p38 phosphorylation was induced by CpG oligonucleotides (ODNs) and LPS in primary salmon mononuclear phagocytes. Pre-treatment of the cells with a p38 inhibitor (SB203580) blocked the PAMP-induced p38 activity and suppressed the upregulation of most of the CpG- and LPS-induced transcripts highlighting the role of this kinase in the salmon innate immune response to PAMPs. In contrast to p38, the phosphorylation of extracellular signal-regulated kinase (ERK), a MAPK involved primarily in response to mitogens, was high in resting cells and, surprisingly, incubation with both CpG and control ODNs downregulated the phospho-ERK levels independently of p38 activation. The basal phospho-ERK level and the CpG-inducible p38 phosphorylation were greatly influenced by the length of in vitro incubation. The basal phospho-ERK level increased gradually throughout a 5-day culture period and was PI3K-dependent as demonstrated by its sensitivity to Wortmannin suggesting it is influenced by growth factors. Overall these data indicate that both basal and PAMP-induced activity of MAPKs might be greatly influenced by the differentiation status of salmon mononuclear phagocytes.en
dc.identifier.citationFish and Shellfish Immunology 35(2013) nr. 4 s. 1079-1085en
dc.identifier.cristinIDFRIDAID 1072659
dc.identifier.doihttp://dx.doi.org710.1016/j.fsi.2013.07.014
dc.identifier.issn1050-4648
dc.identifier.urihttps://hdl.handle.net/10037/5884
dc.identifier.urnURN:NBN:no-uit_munin_5578
dc.language.isoengen
dc.publisherElsevieren
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478en
dc.titleCpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytesen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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