dc.contributor.author | Munang'andu, Hetron Mweemba | |
dc.contributor.author | Fredriksen, Børge Nilsen | |
dc.contributor.author | Mutoloki, Stephen | |
dc.contributor.author | Dalmo, Roy Ambli | |
dc.contributor.author | Evensen, Øystein | |
dc.date.accessioned | 2014-05-09T08:18:12Z | |
dc.date.available | 2014-05-09T08:18:12Z | |
dc.date.issued | 2013 | |
dc.description.abstract | An enduring challenge in the vaccinology of infectious pancreatic necrosis virus (IPNV) is the lack of correlation
between neutralizing antibodies and protection against mortality. To better understand the immunological basis of
vaccine protection, an efficacy trial including Atlantic salmon (Salmo salar L.) vaccinated with a high antigen (HiAg)
or low antigen (LoAg) dose vaccine was carried out in a cohabitation challenge model using the highly virulent
Norwegian Sp strain NVI015. To pinpoint the immunological basis of vaccine protection, pathogenic mechanisms of
IPNV were unraveled in control fish while obtaining feedback on mechanisms of protection in the vaccinated fish.
During the incubation period, infection rates were highest in control fish, followed by the LoAg group with the
lowest infections being in the HiAg group. Although both the liver and pancreas are target organs prone to tissue
damage, infection in the liver was delayed until acute infection in most fish. A correlate of pathology determined as
the cutoff threshold of viral copy numbers linked to tissue damage in target organs was estimated at ≥ 107.0, which
corresponded with an increase in mortality. The kinetics of IFNα and Mx expression suggests that these genes can
be used as biomarkers of IPNV infection progression. Mechanisms of vaccine protection involved reducing infection
rates, preventing infection of the liver and reducing virus replication in target organs to levels below the correlate
of pathology. Overall, the study shows that antigen dose corresponds with vaccine efficacy and that antibody levels
can be used as a signature of protective immunity against pathological disease and mortality. | en |
dc.identifier.citation | Veterinary Research (2013), vol. 44:7 | en |
dc.identifier.cristinID | FRIDAID 1033963 | |
dc.identifier.doi | http://dx.doi.org/10.1186/1297-9716-44-7 | |
dc.identifier.issn | 0928-4249 | |
dc.identifier.uri | https://hdl.handle.net/10037/6219 | |
dc.identifier.urn | URN:NBN:no-uit_munin_5901 | |
dc.language.iso | eng | en |
dc.publisher | BioMed Central | en |
dc.rights.accessRights | openAccess | |
dc.subject | VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 | en |
dc.subject | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 | en |
dc.title | Antigen dose and humoral immune response correspond with protection for inactivated infectious pancreatic necrosis virus vaccines in Atlantic salmon (Salmo salar L) | en |
dc.type | Journal article | en |
dc.type | Tidsskriftartikkel | en |
dc.type | Peer reviewed | en |