Cancer associated fibroblasts in stage I-IIIA NSCLC: Prognostic impact and their correlations with tumor molecular markers
AuthorKilvær, Thomas Karsten; Rakaeekhanehkenari, Mehrdad; Hellevik, Turid; Al-Saad, Samer; Paulsen, Erna-Elise; Busund, Lill-Tove; Dønnem, Tom; Martinez, Inigo Zubiavrre
Methods Tumor specimens from 536 NSCLC patients were obtained and tissue micro-arrays were constructed. Immunohistochemistry was used to evaluate the expression of FAP-1 and α- SMA and explore their impact on survival and association with other tumor molecular markers in NSCLC patients.
Results High expression of FAP-1, but not α-SMA, in squamous cell carcinoma (SCC, P = 0.043, HR = 0.63 95% CI 0.40–0.99) was significantly associated with increased disease-specific survival. FAP-1 and α-SMA were not significantly correlated to each other. Analyses of FAP-1 and α-SMA associated with other tumor-related proteins revealed histotype-specific correlation patterns.
Conclusion The presence of FAP-1 expressing CAFs is an indicator of positive outcome for NSCLCSCC patients. In addition, correlation analyses suggest FAP-1 and α-SMA to label different subsets of fibroblasts and their associations with other tumor-related proteins diverge according to histological subtype.