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dc.contributor.authorOzolina, Agnese
dc.contributor.authorStrike, Eva
dc.contributor.authorNikitina-Zake, Liene
dc.contributor.authorJaunalksne, Inta
dc.contributor.authorKrumina, Angelika
dc.contributor.authorLacis, Romans
dc.contributor.authorBjertnæs, Lars J.
dc.contributor.authorVanags, Indulis
dc.date.accessioned2016-03-08T12:19:12Z
dc.date.available2016-03-08T12:19:12Z
dc.date.issued2015-09-04
dc.description.abstractBackground: Carriers of plasminogen activator inhibitor -1 (PAI-1) -675 genotype 5G/5G may be associated with lower preoperative PAI-1 plasma levels and higher blood loss after heart surgery using cardiopulmonary bypass (CPB). We speculate if polymorphisms of PAI-1 -844 A/G and angiotensin converting enzyme (ACE) intron 16 I/D also might promote fibrinolysis and increase postoperative bleeding. <p>Methods: We assessed PAI-1 -844 A/G, and ACE intron 16 I/D polymorphisms by polymerase chain reaction technique and direct sequencing of genomic DNA from 83 open heart surgery patients that we have presented earlier. As primary outcome, accumulated chest tube drainage (CTD) at 4 and 24 h were analyzed for association with genetic polymorphisms. As secondary outcome, differences in plasma levels of PAI-1, t-PA/PAI-1 complex and D-dimer were determined for each polymorphism. SPSS® was used for statistical evaluation. <p>Results: The lowest preoperative PAI-1 plasma levels were associated with PAI-1 -844 genotype G/G, and higher CTD, as compared with genotype A/A at 4 and 24 h after surgery. Correspondingly, 4 h after the surgery CTD was higher in carriers of ACE intron 16 genotype I/I, as compared with genotype D/D. PAI-1 plasma levels and t-PA/PAI-1 complex reached nadir in carriers of ACE intron 16 genotype I/I, in whom we also noticed the highest D-dimer levels immediately after surgery. Notably, carriers of PAI-1 -844 genotype G/G displayed higher D-dimer levels at 24 h after surgery as compared with those of genotype A/G. <p>Conclusions: Increased postoperative blood loss secondary to enhanced fibrinolysis was associated with carriers of PAI-1 -844 G/G and ACE Intron 16 I/I, suggesting that these genotypes might predict increased postoperative blood loss after cardiac surgery using CPB.en_US
dc.descriptionPublished Version, also available at <a href=http://dx.doi.org/10.1186/s12871-015-0101-1>http://dx.doi.org/10.1186/s12871-015-0101-1</a>en_US
dc.identifier.citationBMC Anesthesiology 2015, 15:122en_US
dc.identifier.issn1471-2253
dc.identifier.otherFRIDAID 1286955
dc.identifier.other10.1186/s12871-015-0101-1
dc.identifier.urihttps://hdl.handle.net/10037/8751
dc.identifier.urnURN:NBN:no-uit_munin_8314
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical molecular biology: 711en_US
dc.titlePolymorphisms on PAI-1 and ACE genes in association with fibrinolytic bleeding after on-pump cardiac surgeryen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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