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dc.contributor.advisorFenton, Kristin
dc.contributor.authorElston, Bryan George Vincent
dc.date.accessioned2016-06-21T09:03:51Z
dc.date.available2016-06-21T09:03:51Z
dc.date.issued2016-05-18
dc.description.abstractIntercellular Adhesion Molecule-1 (ICAM-1, CD54), a cell surface glycoprotein that functions as an integrin, has a well-established role in leukocyte migration during inflammation. Recent studies have indicated a role for this protein in cancer development. In this study three breast cancer cell lines were stimulated with a variety of cytokines. The breast cancer cell lines (MCF7, SK-BR-3 and MDA-MB-468) were chosen to reflect the hormone positive, HER-2 enriched and triple negative subtypes of breast cancer. The cytokines (Interferon gamma, Tumour Necrosis Factor alpha, Interleukin 1beta and Interferon alpha) are commonly encountered in the breast cancer micro-environment. Relative gene expression was measured using quantitative real time polymerase chain reaction. ICAM-1 protein was measured by Western Blot and localised by Immunofluorescence. In addition, ICAM-1 gene expression was measured in human breast cancer samples and ICAM-1 and macrophage (CD68) immunohistochemistry was performed on formalin-fixed, paraffin embedded tumour tissue. ICAM-1 expression was induced in all of the cell lines by IFN, TNF and IL-1 with increased ICAM-1 protein production compared to time-matched controls. IFN appeared to induce ICAM-1 gene expression but no protein was detected. Membranous and para-nuclear ICAM-1 staining was identified after 24 hours in unstimulated MCF7 and MDA-MB-468 cells and the same cell lines stimulated with IFN. ICAM-1 expression was increased in the human tumour tissue compared to normal tissue however this did not correspond with ICAM-1 positivity or numbers of macrophages in the tumour. The study highlights a mechanism that cancer cells may use to enhance their invasive and metastatic potential by making use of a protein normally involved in cell migration.en_US
dc.identifier.urihttps://hdl.handle.net/10037/9326
dc.identifier.urnURN:NBN:no-uit_munin_8892
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.rights.accessRightsopenAccess
dc.rights.holderCopyright 2016 The Author(s)
dc.subject.courseIDMBI-3911
dc.subjectICAM-1en_US
dc.subjectBreast Canceren_US
dc.subjectcell cultureen_US
dc.subjectqPCRen_US
dc.subjectimmunohistochemistryen_US
dc.subjectimmunofluorescenceen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical immunology: 716en_US
dc.titleAn investigation of Intercellular Adhesion Molecule-1 (ICAM-1) in Breast Cnceren_US
dc.typeMaster thesisen_US
dc.typeMastergradsoppgaveen_US


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