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dc.contributor.authorAndreassen, Monica
dc.contributor.authorBøhn, Thomas
dc.contributor.authorWikmark, Odd Gunnar
dc.contributor.authorBodin, Johanna
dc.contributor.authorTraavik, Terje
dc.contributor.authorLøvik, Martinus
dc.contributor.authorNygaard, Unni Cecilie
dc.date.accessioned2016-09-02T10:52:40Z
dc.date.available2016-09-02T10:52:40Z
dc.date.issued2016-05-04
dc.description.abstractBACKGROUND In genetically modified (GM) crops there is a risk that the inserted genes may introduce new allergens and/or adjuvants into the food and feed chain. The MON810 maize, expressing the insecticidal Cry1Ab toxin, is grown in many countries worldwide. In animal models, intranasal and intraperitoneal immunisations with the purified Cry1Ab proteins have induced immune responses, and feeding trials with Cry1Ab-containing feed have revealed some altered immune responses. Previous investigations have primarily measured antibody responses to the protein, while investigations of clinical food allergy symptoms, or allergy promotion (adjuvant effect) associated with the Cry1Ab protein are largely missing. We aimed to investigate immunogenic, allergenic and adjuvant properties of purified Cry1Ab toxin (trypCry1Ab, i.e., trypsin activated Cry1Ab) in a mouse model of food allergy. METHOD Female C3H/HeJ mice were immunized by intragastric gavage of 10 μg purified, trypsin activated Cry1Ab toxin (trypCry1Ab) alone or together with the food allergen lupin. Cholera toxin was added as a positive control for adjuvant effect to break oral tolerance. Clinical symptoms (anaphylaxis) as well as humoral and cellular responses were assessed. RESULTS In contrast to results from previous airway investigations, we observed no indication of immunogenic properties of trypCry1Ab protein after repeated intragastric exposures to one dose, with or without CT as adjuvant. Moreover, the results indicated that trypCry1Ab given by the intragastric route was not able to promote allergic responses or anaphylactic reactions against the co-administered allergen lupin at the given dose. CONCLUSION The study suggests no immunogenic, allergenic or adjuvant capacity of the given dose of trypCry1Ab protein after intragastric exposure of prime aged mice.en_US
dc.description.sponsorshipThe study was funded by GenØk – centre for biosafety and the Norwegian Institute of Public Health.en_US
dc.descriptionPublisher's version, source: <a href=http://doi.org/10.1186/s12865-016-0148-x>http://doi.org/10.1186/s12865-016-0148-x</a>.en_US
dc.identifier.citationBMC Immunology 2016, 17:10en_US
dc.identifier.issn1471-2172
dc.identifier.otherFRIDAID 1354802
dc.identifier.other10.1186/s12865-016-0148-x
dc.identifier.urihttp://hdl.handle.net/10037/9643
dc.identifier.urnURN:NBN:no-uit_munin_9183
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.relation.urihttp://bmcimmunol.biomedcentral.com/articles/10.1186/s12865-016-0148-x
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716en_US
dc.subjectFood allergyen_US
dc.subjectAnaphylaxisen_US
dc.subjectGenetically modified maizeen_US
dc.subjectCry1Aben_US
dc.subjectMiceen_US
dc.titleInvestigations of immunogenic, allergenic and adjuvant properties of Cry1Ab protein after intragastric exposure in a food allergy model in miceen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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