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dc.contributor.authorUsó, Marta
dc.contributor.authorJantus-Lewintre, Eloísa
dc.contributor.authorBremnes, Roy M.
dc.contributor.authorCalabuig, Silvia
dc.contributor.authorBlasco, Ana
dc.contributor.authorPastor, Enrique
dc.contributor.authorBorreda, Irene
dc.contributor.authorMolina-Pinelo, Sonia
dc.contributor.authorPaz-Ares, Luis
dc.contributor.authorGuijarro, Ricardo
dc.contributor.authorMartorell, Miguel
dc.contributor.authorForteza, Jerónimo
dc.contributor.authorCamps, Carlos
dc.contributor.authorSirera, Rafael
dc.date.accessioned2017-03-14T10:24:30Z
dc.date.available2017-03-14T10:24:30Z
dc.date.issued2016
dc.description.abstractThe prognosis of non-small cell lung cancer (NSCLC) remains poor and heterogeneous and new biomarkers are needed. As the immune system plays a pivotal role in cancer, the study of immune-related markers may provide valuable prognostic information of NSCLC. In 122 formalin-fixed, paraffin-embedded tumor tissue samples from early-stage NSCLC, tumor and tumor-near stromal areas were microdissected and gene expression levels of conventional and regulatory T cell markers were assessed by quantitative polymerase chain reaction. Also, the presence of infiltrating CD4+, CD8+, and FOXP3+ cells in tumor samples was assessed by immunohistochemistry. The relative proportion of conventional and regulatory T cells present in the tumor environment was assessed and found to be key to understand the importance that the immune system analysis has in the prognostics of NSCLC patients. The presence of CD8+ cells in the tumor compartment was associated with better outcome, whereas the presence of FOXP3+ cells was associated with worse overall survival. The negative prognostic value of combined biomarkers, indicating high levels of FOXP3 in the stroma and low levels of CD4 or CD8 in tumors, was observed at mRNA level and was validated by immunohistochemistry.In conclusion, the proportion of T helper and cytotoxic cells vs. regulatory T cells in different locations of the tumor microenvironment have opposite prognostic impacts in resected NSCLC.en_US
dc.description.sponsorshipThis work was supported by the Red Temática de Investigación Cooperativa en Cáncer (RD12/0036/0025) and the Fondo de Investigación Sanitaria-Fondo Europeo de Desarrollo Regional (PI09/01147, PI09/01149 and PI12/02838).en_US
dc.descriptionPublished version. Source at <a href=http://doi.org/10.18632/oncotarget.10811>http://doi.org/10.18632/oncotarget.10811</a>. License <a href=https://creativecommons.org/licenses/by/3.0/>CC BY 3.0</a>.en_US
dc.identifier.citationUsó M, Jantus-Lewintre E, Bremnes RM, Calabuig, Blasco A, Pastor, Borreda, Molina-Pinelo, Paz-Ares, Guijarro R, Martorell, Forteza, Camps C, Sirera R. Analysis of the immune microenvironment in resected non-small cell lung cancer: The prognostic value of different T lymphocyte markers. OncoTarget. 2016;7(33):52849-52861en_US
dc.identifier.cristinIDFRIDAID 1395215
dc.identifier.doi10.18632/oncotarget.10811
dc.identifier.issn1949-2553
dc.identifier.urihttps://hdl.handle.net/10037/10638
dc.language.isoengen_US
dc.publisherImpact Journalsen_US
dc.relation.journalOncoTarget
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762en_US
dc.subjectNSCLCen_US
dc.subjectprognosticen_US
dc.subjectimmune-biomarkeren_US
dc.subjecttumor stromaen_US
dc.subjecttumor compartmenten_US
dc.titleAnalysis of the immune microenvironment in resected non-small cell lung cancer: The prognostic value of different T lymphocyte markersen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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