Vis enkel innførsel

dc.contributor.authorSolbakken, Monica Hongrø
dc.contributor.authorTørresen, Ole Kristian
dc.contributor.authorNederbragt, Alexander Johan
dc.contributor.authorSeppola, Marit
dc.contributor.authorGregers, Tone Fredsvik
dc.contributor.authorJakobsen, Kjetill Sigurd
dc.contributor.authorJentoft, Sissel
dc.date.accessioned2017-03-16T14:27:33Z
dc.date.available2017-03-16T14:27:33Z
dc.date.issued2016
dc.description.abstractGenome sequencing of the teleost Atlantic cod demonstrated loss of the Major Histocompatibility Complex (MHC) class II, an extreme gene expansion of MHC class I and gene expansions and losses in the innate pattern recognition receptor (PRR) family of Toll-like receptors (TLR). In a comparative genomic setting, using an improved version of the genome, we characterize PRRs in Atlantic cod with emphasis on TLRs demonstrating the loss of TLR1/6, TLR2 and TLR5 and expansion of TLR7, TLR8, TLR9, TLR22 and TLR25. We find that Atlantic cod TLR expansions are strongly influenced by diversifying selection likely to increase the detectable ligand repertoire through neo- and subfunctionalization. Using RNAseq we find that Atlantic cod TLRs display likely tissue or developmental stage-specific expression patterns. In a broader perspective, a comprehensive vertebrate TLR phylogeny reveals that the Atlantic cod TLR repertoire is extreme with regards to losses and expansions compared to other teleosts. In addition we identify a substantial shift in TLR repertoires following the evolutionary transition from an aquatic vertebrate (fish) to a terrestrial (tetrapod) life style. Collectively, our findings provide new insight into the function and evolution of TLRs in Atlantic cod as well as the evolutionary history of vertebrate innate immunity.en_US
dc.description.sponsorshipThis work was supported by The Research Council of Norway (Grant number 199806/S40 to KSJ). The genome assembly was made using the Abel Cluster, owned by the University of Oslo and the Norwegian metacenter for High Performance Computing (NOTUR), and operated by the Department for Research Computing at USIT, the University of Oslo IT-department. http://www.hpc.uio.no/. The sequencing service was provided by the Norwegian Sequencing Centre (www.sequencing.uio.no), a national technology platform hosted by the University of Oslo and supported by the “Functional Genomics” and “Infrastructure” programs of the Research Council of Norway and the Southeastern Regional Health Authorities.en_US
dc.descriptionPublished version. Source at <a href=http://doi.org/10.1038/srep25211>http://doi.org/10.1038/srep25211</a>. License <a href=https://creativecommons.org/licenses/by/4.0/>CC BY 4.0</a>.en_US
dc.identifier.citationSolbakken et al. Evolutionary redesign of the Atlantic cod (Gadus morhua L.) Toll-like receptor repertoire by gene losses and expansions. Scientific Reports. 2016;6en_US
dc.identifier.issn2045-2322
dc.identifier.otherFRIDAID 1353484
dc.identifier.other10.1038/srep25211
dc.identifier.urihttp://hdl.handle.net/10037/10746
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalScientific Reports
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FundingProgram/179569/Norway///en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FundingProgram/222378/Norway///en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FundingProgram/199806/Norway///en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US
dc.titleEvolutionary redesign of the Atlantic cod (Gadus morhua L.) Toll-like receptor repertoire by gene losses and expansionsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


Tilhørende fil(er)

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel