dc.contributor.author | Solbakken, Monica Hongrø | |
dc.contributor.author | Tørresen, Ole Kristian | |
dc.contributor.author | Nederbragt, Alexander Johan | |
dc.contributor.author | Seppola, Marit | |
dc.contributor.author | Gregers, Tone Fredsvik | |
dc.contributor.author | Jakobsen, Kjetill Sigurd | |
dc.contributor.author | Jentoft, Sissel | |
dc.date.accessioned | 2017-03-16T14:27:33Z | |
dc.date.available | 2017-03-16T14:27:33Z | |
dc.date.issued | 2016 | |
dc.description.abstract | Genome sequencing of the teleost Atlantic cod demonstrated loss of the Major Histocompatibility Complex (MHC) class II, an extreme gene expansion of MHC class I and gene expansions and losses in the innate pattern recognition receptor (PRR) family of Toll-like receptors (TLR). In a comparative genomic setting, using an improved version of the genome, we characterize PRRs in Atlantic cod with emphasis on TLRs demonstrating the loss of TLR1/6, TLR2 and TLR5 and expansion of TLR7, TLR8, TLR9, TLR22 and TLR25. We find that Atlantic cod TLR expansions are strongly influenced by diversifying selection likely to increase the detectable ligand repertoire through neo- and subfunctionalization. Using RNAseq we find that Atlantic cod TLRs display likely tissue or developmental stage-specific expression patterns. In a broader perspective, a comprehensive vertebrate TLR phylogeny reveals that the Atlantic cod TLR repertoire is extreme with regards to losses and expansions compared to other teleosts. In addition we identify a substantial shift in TLR repertoires following the evolutionary transition from an aquatic vertebrate (fish) to a terrestrial (tetrapod) life style. Collectively, our findings provide new insight into the function and evolution of TLRs in Atlantic cod as well as the evolutionary history of vertebrate innate immunity. | en_US |
dc.description.sponsorship | This work was supported by The Research Council of Norway (Grant number 199806/S40 to KSJ). The genome
assembly was made using the Abel Cluster, owned by the University of Oslo and the Norwegian metacenter
for High Performance Computing (NOTUR), and operated by the Department for Research Computing at
USIT, the University of Oslo IT-department. http://www.hpc.uio.no/. The sequencing service was provided by
the Norwegian Sequencing Centre (www.sequencing.uio.no), a national technology platform hosted by the
University of Oslo and supported by the “Functional Genomics” and “Infrastructure” programs of the Research
Council of Norway and the Southeastern Regional Health Authorities. | en_US |
dc.description | Published version. Source at <a href=http://doi.org/10.1038/srep25211>http://doi.org/10.1038/srep25211</a>.
License <a href=https://creativecommons.org/licenses/by/4.0/>CC BY 4.0</a>. | en_US |
dc.identifier.citation | Solbakken et al. Evolutionary redesign of the Atlantic cod (Gadus morhua L.) Toll-like receptor repertoire by gene losses and expansions. Scientific Reports. 2016;6 | en_US |
dc.identifier.cristinID | FRIDAID 1353484 | |
dc.identifier.doi | 10.1038/srep25211 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/10037/10746 | |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature | en_US |
dc.relation.journal | Scientific Reports | |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/FundingProgram/179569/Norway/// | en_US |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/FundingProgram/222378/Norway/// | en_US |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/FundingProgram/199806/Norway/// | en_US |
dc.rights.accessRights | openAccess | en_US |
dc.subject | VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 | en_US |
dc.title | Evolutionary redesign of the Atlantic cod (Gadus morhua L.) Toll-like receptor repertoire by gene losses and expansions | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |