dc.contributor.author | Klein, Jörn | |
dc.contributor.author | Tryland, Morten | |
dc.date.accessioned | 2007-07-27T12:06:10Z | |
dc.date.available | 2007-07-27T12:06:10Z | |
dc.date.issued | 2005-09-05 | |
dc.description.abstract | Background: Two outbreaks of the disease contagious ecthyma were reported in 1999 and 2000
in Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus). Contagious ecthyma is an
epidermal disease of sheep and goats worldwide, which is caused by the zoonotic parapoxvirus orf
virus. Characterisation of clinical samples from the two outbreaks in semi-domesticated reindeer
in Norway by electron microscopy and PCR (B2L) revealed typical parapoxvirus particles and
partial gene sequences corresponding to parapoxvirus, respectively. If contagious ecthyma in
reindeer is caused by orf virus, the virus may be transferred from sheep and goats, via people,
equipment and common use of pastures and corrals, to reindeer. Another possibility is that
contagious ecthyma in reindeer is caused by a hitherto unclassified member of the parapoxvirus
genus that circulates among reindeer herds and remains endemic in Norway.
Results: Genomic comparisons of one standard orf strain (orf NZ2) and the reindeer isolates,
employing restriction fragment length polymorphism (RFLP) and random amplified polymorphic
DNA (RAPD) analysis, demonstrated high similarity between the reindeer viruses and known orf
virus strains. Partial DNA sequences of two different viral genes were determined for the different
isolates and compared with corresponding parapoxvirus genebank sequences. The comparison/
alignment and construction of phylogenetic trees also point to an affiliation of the reindeer viruses
to the species orf virus.
Conclusion: The results of this work imply that the parapoxvirus causing contagious ecthyma in
Norwegian semi-domesticated reindeer belongs to the species orf virus and that the orf virus
crosses the host species barrier from sheep and goat to semi-domesticated reindeer. | en |
dc.format.extent | 1578207 bytes | |
dc.format.mimetype | application/pdf | |
dc.identifier.citation | Virology Journal 2(2005), article no 79 pp 10 | en |
dc.identifier.doi | doi:10.1186/1743-422X-2-79 | |
dc.identifier.issn | 1743-422X | |
dc.identifier.uri | https://hdl.handle.net/10037/1124 | |
dc.identifier.urn | URN:NBN:no-uit_munin_942 | |
dc.language.iso | eng | en |
dc.publisher | BioMed Central | en |
dc.rights.accessRights | openAccess | |
dc.subject | VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical molecular biology: 711 | en |
dc.subject | Medisinsk molekylærbiologi | en |
dc.subject | Virologi | en |
dc.subject | Parapoxviruses | en |
dc.subject | Semi-domesticated | en |
dc.subject | Reindeer | en |
dc.subject | Norway | en |
dc.title | Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus) | en |
dc.type | Journal article | en |
dc.type | Tidsskriftartikkel | en |
dc.type | Peer reviewed | |