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dc.contributor.authorPasing, Yvonne
dc.contributor.authorFenton, Christopher Graham
dc.contributor.authorJorde, Rolf
dc.contributor.authorPaulssen, Ruth H
dc.date.accessioned2018-03-13T10:07:25Z
dc.date.available2018-03-13T10:07:25Z
dc.date.issued2017-03-19
dc.description.abstractVitamin D is hydroxylated in the liver and kidneys to its active form, which can bind to the vitamin D receptor (VDR). The VDR is present in a wide variety of different cells types and tissues and acts as a transcription factor. Although activation of the VDR is estimated to regulate expression of up to 5% of the human genome, our study is the first analysing gene expression after supplementation in more than 10 subjects.<p> Subjects of a randomized controlled trial (RCT) received either vitamin D3 (n = 47) in a weekly dose of 20,000 IU or placebo (n = 47) for a period of three to five years. For this study, blood samples for preparation of RNA were drawn from the subjects and mRNA gene expression in blood was determined using microarray analysis.<p> The two study groups were similar regarding gender, age, BMI and duration of supplementation, whereas the mean serum 25-hydroxyvitamin D (25(OH)D) level as expected was significantly higher in the vitamin D group (119 versus 63 nmol/L). When analysing all subjects, nearly no significant differences in gene expression between the two groups were found. However, when analysing men and women separately, significant effects on gene expression were observed for women. Furthermore, when only including subjects with the highest and lowest serum 25(OH)D levels, additional vitamin D regulated genes were disclosed. Thus, a total of 99 genes (p ≤ 0.05, log2 fold change ≥|0.2|) were found to be regulated, of which 72 have not been published before as influenced by vitamin D. These genes were particularly involved in the interleukin signaling pathway, oxidative stress response, apoptosis signaling pathway and gonadotropin releasing hormone receptor pathway. Thus, our results open the possibility for many future studies.en_US
dc.description.sponsorshipThe North Norway Regional Health Authority The Norwegian Diabetes Association UiT - Arctic University of Norway The Research Council of Norway The Novo Nordisken_US
dc.descriptionAccepted manuscript version. Published version available in <a href=http://doi.org/10.1016/j.jsbmb.2017.03.016> The Journal of Steroid Biochemistry and Molecular Biology (2017) 173, s.93-99. </a>en_US
dc.identifier.citationPasing, Y., Fenton, C.G., Jorde, R. & Paulssen, R.H. (2017). Changes in the human transcriptome upon vitamin D supplementation. Journal of Steroid Biochemistry and Molecular Biology, 173, 93-99.en_US
dc.identifier.cristinIDFRIDAID 1513958
dc.identifier.doi10.1016/j.jsbmb.2017.03.016
dc.identifier.issn0960-0760
dc.identifier.issn1879-1220
dc.identifier.urihttps://hdl.handle.net/10037/12308
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalJournal of Steroid Biochemistry and Molecular Biology
dc.rights.accessRightsopenAccessen_US
dc.subjectApoptosisen_US
dc.subjectGene expressionen_US
dc.subjectFertilityen_US
dc.subjectImmune systemen_US
dc.subjectOxidative stressen_US
dc.subjectVitamin Den_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750en_US
dc.titleChanges in the human transcriptome upon vitamin D supplementationen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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