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dc.contributor.authorCharnock, Colin
dc.contributor.authorSamuelsen, Ørjan
dc.contributor.authorNordlie, Anne-Lise
dc.contributor.authorHjeltnes, Bjarne
dc.date.accessioned2018-03-21T08:02:01Z
dc.date.available2018-03-21T08:02:01Z
dc.date.issued2017-09-22
dc.description.abstractCommercially available microarray (IDENTIBAC AMR-ve) for the detection of antibiotic resistance determinants was investigated for its potential to genotype 30 clinical isolates and two control strains of Klebsiella pneumoniae. Resistance profiles and the production of extended-spectrum b-lactamases were determined by disc diffusion and the results were compared with the microarray profiles in order to assess its scope and limitations. Genes associated with resistance to a wide range of antibiotics, including current first line therapy options, were detected. In addition, the array also detected class 1 integrases. The array is easy to use and interpret, and is useful in providing a general description of the numbers and types of resistance determinants in K. pneumoniae. It also provides an indication of the potential for resistance gene acquisition. However, in most instances detected resistance to specific antibiotics could not unequivocally be assigned to hybridization with a specific array probe. We conclude that the microarray is a valuable and rapid means of investigating the presence of resistance gene classes of therapeutic importance. It can also provide a starting point for selecting analyses of greater resolving power, such as phylogenetic subtyping by PCR sequencing.en_US
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Current Microbiology (2018) 75:163. The final authenticated version is available online at: <a href=https://doi.org/10.1007/s00284-017-1361-4> https://doi.org/10.1007/s00284-017-1361-4 </a>.en_US
dc.identifier.citationCharnock, C., Samuelsen, Ø., Nordlie, A. L. Hjeltnes, B. (2018). Use of a Commercially Available Microarray to Characterize Antibiotic-Resistant Clinical Isolates of Klebsiella pneumoniae. Curr Microbiol 75:163.en_US
dc.identifier.cristinIDFRIDAID 1499503
dc.identifier.issn0343-8651
dc.identifier.issn1432-0991
dc.identifier.otherDOI 10.1007/s00284-017-1361-4
dc.identifier.urihttps://hdl.handle.net/10037/12393
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.journalCurrent Microbiology
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Biofarmasi: 736en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Biopharmacy: 736en_US
dc.titleUse of a Commercially Available Microarray to Characterize Antibiotic-Resistant Clinical Isolates of Klebsiella pneumoniaeen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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