Development of COFRADIC HPLC system for proteomics. Sorting of N-terminal peptides

Abstract

Proteomics, the study of the proteins constituting the proteome, has featured in the past few years as an essential tool in a number of diverse areas of therapeutic research for detection of the initial stage of a disease, for consideration of therapy response and for disintegrating fundamental pathophysiological mechanisms. Proteomics has a wide range of applications such as diabetes research, neurology, nutrition research, diagnosis of renal disease, tumor metastasis, urological cancer research, cardiovascular disease, etc. This context makes proteomics a link between genomics and biology. Proteins can be subjected to posttranslational modifications (PTMs) processes. The results are that the kinds of measured proteins have inter – and intra individual variability. PTMs have a great influence on proteins in the cause of its activity dynamics regulation.