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dc.contributor.authorErkinharju, Toni
dc.contributor.authorDalmo, Roy Ambli
dc.contributor.authorVågsnes, Øyvind
dc.contributor.authorHordvik, Ivar
dc.contributor.authorSeternes, Tore
dc.date.accessioned2018-06-29T12:18:11Z
dc.date.available2018-06-29T12:18:11Z
dc.date.issued2017-12-11
dc.description.abstractWe present a study on the effect of water temperature on immunization of Atlantic lumpfish. In total, 360 fish were vaccinated with either 50 μl of an oil‐based injection vaccine (VAX), with Aeromonas salmonicida and Vibrio salmonicida antigens, or PBS. Fish were vaccinated at three different water temperatures, 5°C, 10°C and 15°C, and sorted into six groups (N = 60). Lumpfish were weighed every 3 weeks after vaccination, sampled at 3, 6, 9 and 18 weeks post‐immunization (wpi) and evaluated by modified Speilberg score, ELISA and immunoblotting. Vaccinated fish showed low antibody response against V. salmonicida. Fish vaccinated at 5°C showed significantly lower antibody response against A. salmonicida throughout the study. At higher temperatures, vaccinated fish showed significantly increased antibody responses, at 18 wpi for 10°C and at 6 and 18 wpi for 15°C. Immunoblotting demonstrated specific response against the LPS antigen of A. salmonicida in the 10°C and 15°C VAX groups. Mean body weight increased in all groups throughout the study. Vaccinated fish had low Speilberg scores with no melanization of abdominal tissue. Our results show that vaccinating lumpfish at a lower water temperature may lead to a low antibody response against A. salmonicida.en_US
dc.description.sponsorshipUiT The Arctic University of Norwayen_US
dc.descriptionThis is the peer reviewed version of the following article: Erkinharju, T., Dalmo, R.A., Vågsnes, Ø., Hordvik, I. & Seternes, T. (2017). Vaccination of Atlantic lumpfish (Cyclopterus lumpus L.) at a low temperature leads to a low antibody response against Aeromonas salmonicida. Journal of Fish Diseases, 612-623, which has been published in final form at <a href=https://doi.org/10.1111/jfd.12760> https://doi.org/10.1111/jfd.12760</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en_US
dc.identifier.citationErkinharju, T., Dalmo, R.A., Vågsnes, Ø., Hordvik, I. & Seternes, T. (2017). Vaccination of Atlantic lumpfish (Cyclopterus lumpus L.) at a low temperature leads to a low antibody response against Aeromonas salmonicida. Journal of Fish Diseases, 612-623. https://doi.org/10.1111/jfd.12760en_US
dc.identifier.cristinIDFRIDAID 1527015
dc.identifier.doi10.1111/jfd.12760
dc.identifier.issn0140-7775
dc.identifier.issn1365-2761
dc.identifier.urihttps://hdl.handle.net/10037/13061
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalJournal of Fish Diseases
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/239140/Norway/Nanoparticle encapsulation of plant-based vaccine against piscine reovirus infection in salmon//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectAeromonas salmonicidaen_US
dc.subjectAtlantic lumpfishen_US
dc.subjectimmune responseen_US
dc.subjectside effecten_US
dc.subjecttemperatureen_US
dc.subjectvaccineen_US
dc.titleVaccination of Atlantic lumpfish (Cyclopterus lumpus L.) at a low temperature leads to a low antibody response against Aeromonas salmonicidaen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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