ub.xmlui.mirage2.page-structure.muninLogoub.xmlui.mirage2.page-structure.openResearchArchiveLogo
    • EnglishEnglish
    • norsknorsk
  • Velg spraaknorsk 
    • EnglishEnglish
    • norsknorsk
  • Administrasjon/UB
Vis innførsel 
  •   Hjem
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Norges fiskerihøgskole
  • Artikler, rapporter og annet (Norges fiskerihøgskole)
  • Vis innførsel
  •   Hjem
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Norges fiskerihøgskole
  • Artikler, rapporter og annet (Norges fiskerihøgskole)
  • Vis innførsel
JavaScript is disabled for your browser. Some features of this site may not work without it.

Profiling Atlantic salmon B cell populations: CpG-mediated TLR-ligation enhances IgM secretion and modulates immune gene expression

Permanent lenke
https://hdl.handle.net/10037/13446
DOI
https://doi.org/10.1038/s41598-018-21895-9
Thumbnail
Åpne
article.pdf (1.672Mb)
Publisher's version (PDF)
Dato
2018-02-23
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Forfatter
Jenberie, Shiferaw; Thim, Hanna Leena; Sunyer, J Oriol; Skjødt, Karsten; Jensen, Ingvill; Jørgensen, Jorunn B
Sammendrag
While TLR-activated pathways are key regulators of B cell responses in mammals, their impact on teleost B cells are scarcely addressed. Here, the potential of Atlantic salmon B cells to respond to TLR ligands was shown by demonstrating a constitutive expression of nucleic-acid sensing TLRs in magnetic sorted IgM+ cells. Of the two receptors recognizing CpG in teleosts, tlr9 was the dominating receptor with over ten-fold higher expression than tlr21. Upon CpG-stimulation, IgM secretion increased for head kidney (HK) and splenic IgM+ cells, while blood B cells were marginally affected. The results suggest that CpG directly affects salmon B cells to differentiate into antibody secreting cells (ASCs). IgM secretion was also detected in the non-treated controls, again with the highest levels in the HK derived population, signifying that persisting ASCs are present in this tissue. In all tissues, the IgM+ cells expressed high MHCII levels, suggesting antigen-presenting functions. Upon CpG-treatment the co-stimulatory molecules cd83 and cd40 were upregulated, while cd86 was down-regulated under the same conditions. Finally, ifna1 was upregulated upon CpG-stimulation in all tissues, while a restricted upregulation was evident for ifnb, proposing that salmon IgM+ B cells exhibit a type I IFN-response.
Beskrivelse
Source at https://doi.org/10.1038/s41598-018-21895-9. Licensed CC BY-NC-ND 4.0.
Er en del av
Jenberie, S. (2020). Atlantic salmon B cells- local and systemic responses to intraperitoneally administered salmonid alphavirus. (Doctoral thesis). https://hdl.handle.net/10037/17566.
Forlag
Nature Publishing Group
Sitering
Jenberie, S., Thim, H.L., Sunyer, J.O., Skjødt, K., Jensen, I. & Jørgensen, J.B. (2018). Profiling Atlantic salmon B cell populations: CpG-mediated TLR-ligation enhances IgM secretion and modulates immune gene expression. Scientific Reports, 8(3565). https://doi.org/10.1038/s41598-018-21895-9
Metadata
Vis full innførsel
Samlinger
  • Artikler, rapporter og annet (Norges fiskerihøgskole) [1053]

Bla

Bla i hele MuninEnheter og samlingerForfatterlisteTittelDatoBla i denne samlingenForfatterlisteTittelDato
Logg inn

Statistikk

Antall visninger
UiT

Munin bygger på DSpace

UiT Norges Arktiske Universitet
Universitetsbiblioteket
uit.no/ub - munin@ub.uit.no

Tilgjengelighetserklæring