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dc.contributor.authorJenberie, Shiferaw
dc.contributor.authorThim, Hanna Leena
dc.contributor.authorSunyer, J Oriol
dc.contributor.authorSkjødt, Karsten
dc.contributor.authorJensen, Ingvill
dc.contributor.authorJørgensen, Jorunn B
dc.date.accessioned2018-08-17T07:19:16Z
dc.date.available2018-08-17T07:19:16Z
dc.date.issued2018-02-23
dc.description.abstractWhile TLR-activated pathways are key regulators of B cell responses in mammals, their impact on teleost B cells are scarcely addressed. Here, the potential of Atlantic salmon B cells to respond to TLR ligands was shown by demonstrating a constitutive expression of nucleic-acid sensing TLRs in magnetic sorted IgM<sup>+</sup> cells. Of the two receptors recognizing CpG in teleosts, <i>tlr9</i> was the dominating receptor with over ten-fold higher expression than <i>tlr21</i>. Upon CpG-stimulation, IgM secretion increased for head kidney (HK) and splenic IgM<sup>+</sup> cells, while blood B cells were marginally affected. The results suggest that CpG directly affects salmon B cells to differentiate into antibody secreting cells (ASCs). IgM secretion was also detected in the non-treated controls, again with the highest levels in the HK derived population, signifying that persisting ASCs are present in this tissue. In all tissues, the IgM<sup>+</sup> cells expressed high MHCII levels, suggesting antigen-presenting functions. Upon CpG-treatment the co-stimulatory molecules <i>cd83</i> and <i>cd40</i> were upregulated, while <i>cd86</i> was down-regulated under the same conditions. Finally, <i>ifna1</i> was upregulated upon CpG-stimulation in all tissues, while a restricted upregulation was evident for <i>ifnb</i>, proposing that salmon IgM<sup>+</sup> B cells exhibit a type I IFN-response.en_US
dc.description.sponsorshipUiT the Arctic University of Norway The National Institutes of Health Grant The National Science Foundation Granten_US
dc.descriptionSource at <a href=https://doi.org/10.1038/s41598-018-21895-9> https://doi.org/10.1038/s41598-018-21895-9</a>. Licensed <a href=http://creativecommons.org/licenses/by-nc-nd/4.0/> CC BY-NC-ND 4.0. </a>en_US
dc.identifier.citationJenberie, S., Thim, H.L., Sunyer, J.O., Skjødt, K., Jensen, I. & Jørgensen, J.B. (2018). Profiling Atlantic salmon B cell populations: CpG-mediated TLR-ligation enhances IgM secretion and modulates immune gene expression. Scientific Reports, 8(3565). https://doi.org/10.1038/s41598-018-21895-9en_US
dc.identifier.cristinIDFRIDAID 1571761
dc.identifier.doi10.1038/s41598-018-21895-9
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/13446
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofJenberie, S. (2020). Atlantic salmon B cells- local and systemic responses to intraperitoneally administered salmonid alphavirus. (Doctoral thesis). <a href=https://hdl.handle.net/10037/17566>https://hdl.handle.net/10037/17566</a>.
dc.relation.journalScientific Reports
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/237315/Norway/Fish Virus Vaccines/ViVaFish/en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.titleProfiling Atlantic salmon B cell populations: CpG-mediated TLR-ligation enhances IgM secretion and modulates immune gene expressionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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