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Faecal colonization of E. coli and Klebsiella spp. producing extended-spectrum beta-lactamases and plasmid-mediated AmpC in Mozambican university students

Permanent lenke
https://hdl.handle.net/10037/13976
DOI
https://doi.org/10.1186/s12879-018-3154-1
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article.pdf (925.7Kb)
Publisher's version (PDF)
Dato
2018-01-08
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Forfatter
Chirindze, Lourenco Marcos; Zimba, Tomas F.; Sekyere, John Osei; Govinden, Usha; Chenia, Hafizah Y.; Sundsfjord, Arnfinn; Essack, Sabiha Y.; Simonsen, Gunnar Skov
Sammendrag

Background: In recent years, the world has seen a surge in Enterobacteriaceae resistant to broad-spectrum beta-lactam antibiotics due to the production of extended-spectrum beta-lactamases (ESBLs) or plasmid-mediated AmpC (pAmpC) enzymes. Data on the epidemiology of cephalosporin-resistant Enterobacteriaceae in Sub-Saharan Africa are still limited.

Methods: Two hundred seventy-five non-repetitive stool samples were collected from Mozambican university students of both sexes. Samples were cultured on MacConkey agar with and without ceftriaxone (1 mg/L) for selection of third-generation cephalosporin-resistant isolates, which were subjected to antimicrobial susceptibility testing by disc diffusion, characterization of resistance genes by PCR and ERIC-PCR analysis for strain clonality.

Results: Among the 275 students, 55 (20%) carried a total of 56 E. coli (n = 35) and Klebsiella spp. (n = 21) isolates resistant to ceftriaxone and phenotypically positive for ESBL- and/or pAmpC-production. Forty-three percent of the isolates (24/56) contained only ESBL genes, 11% (6/56) only pAmpC genes, and 36% (20/56) both ESBL and pAmpC genes. The remaining six isolates were negative for the CTX-M/pAmpC genes included in the test panel. E. coli and Klebsiella spp. combined demonstrated 70% resistance to tetracycline and co-trimoxazole, 63% to ceftazidime and 34% to ciprofloxacin. In total, 89% of ESBL/pAmpC-positive isolates were defined as multi-resistant by being resistant to three or more antibiotic classes. ERIC-PCR fingerprinting demonstrated low similarity among isolates. None of the participants reported recent hospitalization and just 12.5% had taken antibiotics 3 months prior to the study.

Conclusion: This study demonstrated 20% colonization with multi-resistant E. coli and Klebsiella spp. among Mozambican students with a diversity of ESBL and pAmpC genes. Colonization was not related to prior hospitalization or antimicrobial consumption.

Beskrivelse
Source at https://doi.org/10.1186/s12879-018-3154-1. Licensed CC BY-NC-ND 4.0.
Forlag
BMC
Sitering
Chirindze, L.M., Zimba, T.F., Sekyere, J.O., Govinden, U., Chenia, H.Y., Sundsfjord, A., ... Simonsen, G.S. (2018). Faecal colonization of E. coli and Klebsiella spp. producing extended-spectrum beta-lactamases and plasmid-mediated AmpC in Mozambican university students. BMC Infectious Diseases, 18(244). https://doi.org/10.1186/s12879-018-3154-1
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