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dc.contributor.advisorHansen, Terkel
dc.contributor.advisorPasing, Yvonne
dc.contributor.advisorSchniers, Armin
dc.contributor.authorSivertsen, Christoffer Ågnes
dc.date.accessioned2019-03-05T09:51:51Z
dc.date.available2019-03-05T09:51:51Z
dc.date.issued2018-05-15
dc.description.abstractBackground: Identifying modifications made to terminal parts of proteins are very useful in understanding diseases and other out of the ordinary biological states. This thesis has focused on developing methods for enriching N-terminal and C-terminal peptides from a complex protein mixture, so that analysis of these samples can give better, more comprehensive and more reproducible results.en_US
dc.description.abstractMaterials and methods: This thesis applies a bottom-up proteomics work-flow approach to develop and compare methods for enrichment of N-terminal, using two digestion enzymes, three sample clean-up methods, and two enrichment agents in different combinations. One method for C-terminal enrichment was developed, with basis in the method that gave the best results from N-terminal enrichment.en_US
dc.description.abstractResults and discussion: None of the N-terminal enrichment methods improved the number of terminal peptides compared to the control samples. However, the results suggest that trypsin should be the enzyme of choice when enriching for N-terminal. The method for enrichment of C-terminal peptides was developed with basis in the method that gave the best results for the N-terminal enrichment. This method yielded only one terminal peptide, which is far lower than expected based on existing literature.en_US
dc.description.abstractConclusion: While none of the methods developed in this thesis improves the number of terminal peptides, it would seem that trypsin should be chosen over chymotrypsin when enriching N-terminal peptides. This result, however, has not been validated and should be investigated further.en_US
dc.identifier.urihttps://hdl.handle.net/10037/14835
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2018 The Author(s)
dc.subject.courseIDFAR-3911
dc.subjectVDP::Technology: 500::Biotechnology: 590en_US
dc.subjectVDP::Teknologi: 500::Bioteknologi: 590en_US
dc.titleIdentification of N-terminal and C-terminal peptides in proteomicsen_US
dc.typeMaster thesisen_US
dc.typeMastergradsoppgaveen_US


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