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dc.contributor.authorJamaly, Simin
dc.contributor.authorRamberg, Cathrine
dc.contributor.authorOlsen, Randi
dc.contributor.authorLatysheva, Nadezhda
dc.contributor.authorWebster, Paul
dc.contributor.authorSovershaev, Timofey
dc.contributor.authorBrækkan, Sigrid Kufaas
dc.contributor.authorHansen, John-Bjarne
dc.date.accessioned2019-03-12T08:42:31Z
dc.date.available2019-03-12T08:42:31Z
dc.date.issued2018-11-21
dc.description.abstractOptimal pre-analytical handling is essential for valid measurements of plasma concentration and size distribution of extracellular vesicles (EVs). We investigated the impact of plasma preparation, various anticoagulants (Citrate, EDTA, CTAD, Heparin), and fasting status on concentration and size distribution of EVs measured by Nanoparticle Tracking Analysis (NTA). Blood was drawn from 10 healthy volunteers to investigate the impact of plasma preparation and anticoagulants, and from 40 individuals from a population-based study to investigate the impact of postprandial lipidemia. Plasma concentration of EVs was measured by NTA after isolation by high-speed centrifugation, and size distribution of EVs was determined using NTA and scanning electron microscopy (SEM). Plasma concentrations and size distributions of EVs were essentially similar for the various anticoagulants. Transmission electron microscopy (TEM) confrmed the presence of EVs. TEM and SEM-analyses showed that the EVs retained spherical morphology after high-speed centrifugation. Plasma EVs were not changed in postprandial lipidemia, but the mean sizes of VLDL particles were increased and interfered with EV measurements (explained 66% of the variation in EVs-concentration in the postprandial phase). Optimization of procedures for separating VLDL particles and EVs is therefore needed before NTAassessment of EVs can be used as biomarkers of disease.en_US
dc.description.sponsorshipStifelsen Kristian Gerhard Jebsenen_US
dc.identifier.citationJamaly, S., Ramberg, C., Olsen, R., Latysheva, N., Webster, P., Sovershaev, T, ... Hansen, J.B. (2018). Impact of preanalytical conditions on plasma concentration and size distribution of extracellular vesicles using Nanoparticle Tracking Analysis. <i>Scientific Reports</i>, 8:17216. https://doi.org/10.1038/s41598-018-35401-8en_US
dc.identifier.cristinIDFRIDAID 1673303
dc.identifier.doi10.1038/s41598-018-35401-8
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/14945
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.relation.ispartofRamberg, C. (2021). The Role of Plasma Extracellular Vesicles and Procoagulant Phospholipid Activity in Venous Thromboembolism. (Doctoral thesis). <a href=https://hdl.handle.net/10037/22767>https://hdl.handle.net/10037/22767</a>.
dc.relation.journalScientific Reports
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Authors
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleImpact of preanalytical conditions on plasma concentration and size distribution of extracellular vesicles using Nanoparticle Tracking Analysisen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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