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dc.contributor.authorPedersen, Tina Myhre
dc.contributor.authorBoardman, Neoma
dc.contributor.authorHafstad, Anne Dragøy
dc.contributor.authorAasum, Ellen
dc.date.accessioned2019-03-18T13:02:03Z
dc.date.available2019-03-18T13:02:03Z
dc.date.issued2018-10-01
dc.description.abstractAlthough murine models for studying the development of cardiac dysfunction in diabetes mellitus are well established, their reported cardiac phenotypes vary. These reported divergences may, in addition to the severity of different models, also be linked to the methods used for cardiac functional assessment. In the present study, we examined the functional changes using conventional transthoracic echocardiography (<i>in vivo</i>) and isolated heart perfusion techniques (<i>ex vivo</i>), in hearts from two mouse models; one with an overt type 2 diabetes (the <i>db/db</i> mouse) and one with a prediabetic state, where obesity was induced by a high-fat diet (HFD). Analysis of left ventricular function in the isolated working hearts from HFD-fed mice, suggested that these hearts develop diastolic dysfunction with preserved systolic function. Accordingly, <i>in vivo</i> examination demonstrated maintained systolic function, but we did not find parameters of diastolic function to be altered. In <i>db/db</i> mice, <i>ex vivo</i> working hearts showed both diastolic and systolic dysfunction. Although <i>in vivo</i> functional assessment revealed signs of diastolic dysfunction, the hearts did not display reduced systolic function. The contrasting results between <i>ex vivo</i> and <i>in vivo</i> function could be due to systemic changes that may sustain <i>in vivo</i> function, or a lack of sensitivity using conventional transthoracic echocardiography. Thus, this study demonstrates that the isolated perfused working heart preparation provides unique additional information related to the development of cardiomyopathy, which might otherwise go unnoticed when only using conventional echocardiographic assessment.en_US
dc.description.sponsorshipThe Norwegian Council on Cardiovascular Disease UiT - The Arctic University of Norwayen_US
dc.descriptionSource at <a href=https://doi.org/10.1371/journal.pone.0204843>https://doi.org/10.1371/journal.pone.0204843. </a>en_US
dc.identifier.citationPedersen, T.M., Boardman, N.T., Hafstad, A.D. & Aasum, E. (2018). Isolated perfused working hearts provide valuable additional information during phenotypic assessment of the diabetic mouse heart. <i>PLoS ONE, 13</i>(10), e0204843. https://doi.org/10.1371/journal.pone.0204843en_US
dc.identifier.cristinIDFRIDAID 1627437
dc.identifier.doi10.1371/journal.pone.0204843
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/15015
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.journalPLoS ONE
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleIsolated perfused working hearts provide valuable additional information during phenotypic assessment of the diabetic mouse hearten_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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