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dc.contributor.authorPain, Maria Charlene Ronessen
dc.contributor.authorHjerde, Erik
dc.contributor.authorKlingenberg, Claus
dc.contributor.authorCavanagh, Jorunn Pauline
dc.date.accessioned2020-02-25T14:37:23Z
dc.date.available2020-02-25T14:37:23Z
dc.date.issued2019-09-10
dc.description.abstract<i>Staphylococcus haemolyticus</i> is a skin commensal gaining increased attention as an emerging pathogen of nosocomial infections. However, knowledge about the transition from a commensal to an invasive lifestyle remains sparse and there is a paucity of studies comparing pathogenicity traits between commensal and clinical isolates. In this study, we used a pan-genomic approach to identify factors important for infection and hospital adaptation by exploring the genomic variability of 123 clinical isolates and 46 commensal <i>S. haemolyticus</i> isolates. Phylogenetic reconstruction grouped the 169 isolates into six clades with a distinct distribution of clinical and commensal isolates in the different clades. Phenotypically, multi-drug antibiotic resistance was detected in 108/123 (88%) of the clinical isolates and 5/46 (11%) of the commensal isolates (<i>p</i> < 0.05). In the clinical isolates, we commonly identified a homolog of the serine-rich repeat glycoproteins <i>sraP</i>. Additionally, three novel capsular polysaccharide operons were detected, with a potential role in <i>S. haemolyticus</i> virulence. Clinical <i>S. haemolyticus</i> isolates showed specific signatures associated with successful hospital adaption. Biofilm forming <i>S. haemolyticus</i> isolates that are resistant to oxacillin (<i>mecA</i>) and aminoglycosides (<i>aacA-aphD</i>) are most likely invasive isolates whereas absence of these traits strongly indicates a commensal isolate. We conclude that our data show a clear segregation of isolates of commensal origin, and specific genetic signatures distinguishing the clinical isolates from the commensal isolates. The widespread use of antimicrobial agents has probably promoted the development of successful hospital adapted clones of <i>S. haemolyticus</i> clones through acquisition of mobile genetic elements or beneficial point mutations and rearrangements in surface associated genes.en_US
dc.identifier.citationPain MC, Hjerde e, Klingenberg C, Cavanagh JP. Comparative Genomic Analysis of Staphylococcus haemolyticus Reveals Key to Hospital Adaptation and Pathogenicity. Frontiers in Microbiology. 2019;10en_US
dc.identifier.cristinIDFRIDAID 1739604
dc.identifier.doi10.3389/fmicb.2019.02096
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/10037/17501
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.ispartofPain, M.C.R. (2020). Exploring the pangenome of Staphylococcus haemolyticus. Colonisation, hospital adaption, pathogenicity and novel species identification. (Doctoral thesis). <a href=https://hdl.handle.net/10037/17894>https://hdl.handle.net/10037/17894. </a>
dc.relation.journalFrontiers in Microbiology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical microbiology: 715en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715en_US
dc.titleComparative Genomic Analysis of Staphylococcus haemolyticus Reveals Key to Hospital Adaptation and Pathogenicityen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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