ub.xmlui.mirage2.page-structure.muninLogoub.xmlui.mirage2.page-structure.openResearchArchiveLogo
    • EnglishEnglish
    • norsknorsk
  • Velg spraaknorsk 
    • EnglishEnglish
    • norsknorsk
  • Administrasjon/UB
Vis innførsel 
  •   Hjem
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Norges fiskerihøgskole
  • Artikler, rapporter og annet (Norges fiskerihøgskole)
  • Vis innførsel
  •   Hjem
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Norges fiskerihøgskole
  • Artikler, rapporter og annet (Norges fiskerihøgskole)
  • Vis innførsel
JavaScript is disabled for your browser. Some features of this site may not work without it.

T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis

Permanent lenke
https://hdl.handle.net/10037/17710
DOI
https://doi.org/10.1016/j.fsi.2019.07.021
Thumbnail
Åpne
article.pdf (1.961Mb)
Akseptert manusversjon , licensed CC BY-NC-ND (PDF)
Dato
2019-07-11
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Forfatter
Chi, Heng; Sørmo, Kristian Gillebo; Diao, Jing; Dalmo, Roy Ambli
Sammendrag
Eomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which possesses a TBOX_3 domain similar to its counterpart in mammals. The regulated expression was observed in head kidney and spleen in response to live Vibrio anguillarum infection in vivo, and spleen leukocytes in vitro after PMA and poly I:C stimulation. Furthermore, we determined a 694 bp sequence, upstream of the transcriptional start site (TSS), to contain a number of sequence motifs that matched known transcription factor-binding sites. Activities of the presumptive regulatory gene were assessed by transfecting different 5′-deletion constructs in CHSE-214 cells. The results showed that the basal promoters and positive transcriptional regulator activities of GmEomes were dependent by sequences located from −694 to −376 bp upstream of TSS. Furthermore, we found that some Eomes binding sites were present in the 5′-flanking regions of the cod IFNγ gene predicted by bioinformatics. However, Co-transfection of eomesodermin overexpression plasmids with INFγ reporter vector into CHSE-214 cells determined that Atlantic cod eomesodermin played a minor role in activation of the INFγ promoter.
Forlag
Elsevier
Sitering
Chi H, Sørmo KG, Diao, Dalmo RA. T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis. Fish and Shellfish Immunology. 2019;93:28-38
Metadata
Vis full innførsel
Samlinger
  • Artikler, rapporter og annet (Norges fiskerihøgskole) [1053]
Copyright 2019 The Author(s).

Bla

Bla i hele MuninEnheter og samlingerForfatterlisteTittelDatoBla i denne samlingenForfatterlisteTittelDato
Logg inn

Statistikk

Antall visninger
UiT

Munin bygger på DSpace

UiT Norges Arktiske Universitet
Universitetsbiblioteket
uit.no/ub - munin@ub.uit.no

Tilgjengelighetserklæring