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dc.contributor.authorRasmussen, Mads Skytte
dc.contributor.authorBirgisdottir, Åsa birna
dc.contributor.authorJohansen, Terje
dc.date.accessioned2020-03-11T16:33:08Z
dc.date.available2020-03-11T16:33:08Z
dc.date.issued2019-01-05
dc.description.abstractThe mammalian ATG8 proteins (LC3A-C/GABARAP, GABARAPL1, and GABARAPL2) are small ubiquitin-like proteins critically involved in macroautophagy. Their processed C-termini are posttranslationally conjugated to a phosphatidylethanolamine moiety, enabling their insertion into the lipid bilayers of both the inner and outer membranes of the forming autophagosomes. The ATG8s bind a diverse selection of proteins including cargo receptors for selective autophagy, members of the core autophagy machinery, and other proteins involved in formation, transport, and maturation (fusion to lysosomes) of autophagosomes. Protein binding to the ATG8s is in most cases mediated by short, conserved sequence motifs known as LC3-interacting regions (LIRs). Here, we present a protocol for identifying putative LIR motifs in a whole protein sequence using peptide arrays generated by SPOT synthesis on nitrocellulose membranes. The use of two-dimensional peptide arrays allows for further identification of specific residues critical for LIR binding.en_US
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-8873-0_8en_US
dc.identifier.citationRasmussen, M.S., Birgisdottir, Å.b., Johansen, T. (2019) Use of Peptide Arrays for Identification and Characterization of LIR Motifs. <i> Methods in molecular biology, 1880, </i> 149-161en_US
dc.identifier.cristinIDFRIDAID 1696998
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttps://hdl.handle.net/10037/17721
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalMethods in molecular biology
dc.relation.projectIDNorges forskningsråd: 214448en_US
dc.relation.projectIDKreftforeningen: 71043-PR-2006-0320en_US
dc.relation.projectIDNorges forskningsråd: 196898en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© Springer Science+Business Media, LLC, part of Springer Nature 2019en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473en_US
dc.titleUse of Peptide Arrays for Identification and Characterization of LIR Motifsen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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