Show simple item record

dc.contributor.authorStiksrud, Birgitte
dc.contributor.authorAass, Hans Christian Dalsbotten
dc.contributor.authorLorvik, Kristina Berg
dc.contributor.authorUeland, Thor
dc.contributor.authorTrøseid, Marius
dc.contributor.authorDyrhol-Riise, Anne Ma
dc.date.accessioned2020-03-17T13:09:17Z
dc.date.available2020-03-17T13:09:17Z
dc.date.issued2019-06-01
dc.description.abstract<i>Objective</i>: To explore monocyte and dendritic cell immune responses, and their association with future CD4<sup>+</sup> gain in treated HIV patients with suboptimal CD4<sup>+</sup> recovery.<p> <p><i>Design</i>: A cross-sectional study of HIV-infected, virally suppressed individuals on antiretroviral therapy for at least 24 months; 41 immunological nonresponders (INRs) (CD4+ cell count <400 cells/μl) and 26 immunological responders (CD4<sup>+</sup> cell count >600 cells/μl). Ten HIV-infected antiretroviral therapy-naive and 10 HIV-negative healthy persons served as controls. CD4<sup>+</sup> cell counts were registered after median 2.4 and 4.7 years.<p> <p><i>Methods</i>: Monocyte, dendritic-cell and T-cell activation and regulatory T cells (Tregs) were analyzed by flow cytometry. In INR and immunological responder subgroups matched on age and nadir CD4<sup>+</sup> cell count, upregulation of interferon-inducible protein-10 (IP-10) and indoleamine 2,3-dioxygenase in monocytes and dendritic cells and cytokines in cell supernatants were measured <i>in vitro</i> in peripheral blood mononuclear cells stimulated with aldrithiol-2-inactivated HIV-1.<p> <p><i>Results</i>: The INR group displayed higher spontaneous activation of both monocytes (HLA-DR<sup>+</sup>) and myeloid and plasmacytoid dendritic cells (HLA-DR<sup>+</sup>, CD83<sup>+</sup> and CD86<sup>+</sup>) compared with immunological responders, and this was associated with increased T-cell activation (CD38<sup>+</sup>HLA-DR<sup>+</sup>), an effector memory T-cell phenotype and activated Tregs. The IP-10 response in monocytes after in-vitro HIV stimulation was negatively associated with prospective CD4<sup>+</sup> gain. IP-10, indoleamine 2,3-dioxygenase and cytokines levels were comparable between the groups, but inversely correlated with activated Tregs in INRs.<p> <p><i>Conclusion</i>: HIV-infected individuals with suboptimal immune recovery demonstrated more activated monocytes and in particular dendritic cells, compared with patients with acceptable CD4<sup>+</sup> gain. A high level of HIV-specific IP-10 expression in monocytes may be predictive of future CD4<sup>+</sup> recovery.en_US
dc.identifier.citationStiksrud B, Aass HC, Lorvik KB, Ueland T, Trøseid M, Dyrhol-Riise AM. Activated dendritic cells and monocytes in HIV immunological nonresponders: HIV-induced interferon-inducible protein-10 correlates with low future CD4+ recovery. AIDS (London). 2019;33(7):1117-1129en_US
dc.identifier.cristinIDFRIDAID 1714251
dc.identifier.doi10.1097/QAD.0000000000002173
dc.identifier.issn0269-9370
dc.identifier.issn1473-5571
dc.identifier.urihttps://hdl.handle.net/10037/17770
dc.language.isoengen_US
dc.publisherLippincott, Williams & Wilkinsen_US
dc.relation.journalAIDS (London)
dc.relation.projectIDNorges forskningsråd: 144182en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/?/144182?/Norway/?/?/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleActivated dendritic cells and monocytes in HIV immunological nonresponders: HIV-induced interferon-inducible protein-10 correlates with low future CD4+ recoveryen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record