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Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake

Permanent lenke
https://hdl.handle.net/10037/18550
DOI
https://doi.org/10.1038/s41467-020-14343-8
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article.pdf (6.297Mb)
Publisert versjon (PDF)
Dato
2020-01-23
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Forfatter
Jakobi, Arjen J.; Huber, Stefan T.; Mortensen, Simon A.; Schultz, Sebastian; Palara, Anthimi; Kuhm, Tanja; Shrestha, Birendra Kumar; Lamark, Trond; Hagen, Wim J.H.; Wilmanns, Matthias; Johansen, Terje; Brech, Andreas; Sachse, Carsten
Sammendrag
p62/SQSTM1 is an autophagy receptor and signaling adaptor with an N-terminal PB1 domain that forms the scaffold of phase-separated p62 bodies in the cell. The molecular determinants that govern PB1 domain filament formation in vitro remain to be determined and the role of p62 filaments inside the cell is currently unclear. We here determine four high-resolution cryo-EM structures of different human and Arabidopsis PB1 domain assemblies and observed a filamentous ultrastructure of p62/SQSTM1 bodies using correlative cellular EM. We show that oligomerization or polymerization, driven by a double arginine finger in the PB1 domain, is a general requirement for lysosomal targeting of p62. Furthermore, the filamentous assembly state of p62 is required for autophagosomal processing of the p62-specific cargo KEAP1. Our results show that using such mechanisms, p62 filaments can be critical for cargo uptake in autophagy and are an integral part of phase-separated p62 bodies.
Er en del av
Palara, A. (2021). Mechanism and structural requirements for formation of p62 bodies and degradation of p62 by selective autophagy. (Doctoral thesis). https://hdl.handle.net/10037/22992
Forlag
Nature Research
Sitering
Jakobi, Huber, Mortensen, Schultz S, Palara A, Kuhm, Shrestha BK, Lamark T, Hagen WJ, Wilmanns M, Johansen T, Brech A, Sachse C. Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake. Nature Communications. 2020;11:440:1-15
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  • Artikler, rapporter og annet (medisinsk biologi) [1103]
Copyright 2020 The Author(s)

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